Scholarlink

<p>Tannery wastewater presents a significant challenge for biological treatment due to its high concentrations of nitrogen and sulfide. Conventional heterotrophic denitrification is often limited by the requirement for costly organic carbon supplementation. This study demonstrates efficient nitrogen removal from authentic tannery wastewater without external carbon input, achieved under synergistic mixotrophic conditions. Integrated multi-omics analyses revealed sulfur autotrophic denitrification (SADN) as the primary nitrogen removal pathway. Concurrently, endogenous heterotrophs (e.g.,&#xa0;<i>Bacteroidota</i>) degraded inherent organic matter, contributing to chemical oxygen demand (COD) removal. By integrating multi-omics analyses (16S rRNA sequencing, metagenomics, and metaproteomics), we uncovered the underlying microbial synergy. The process was facilitated by a succession of functional microorganisms, predominantly&#xa0;<i>Proteobacteria</i>&#xa0;and&#xa0;<i>Bacteroidota</i>, which facilitated simultaneous sulfur oxidation and organic matter degradation. We identified a complete enzymatic electron transfer chain, coupling sulfur oxidation with denitrification, which was strongly supported by the co-expression of key relevant genes and the high abundance of their corresponding core enzymes. This microbial synergy resulted in a substantially enhanced total nitrogen removal rate without any organic carbon input, alongside a notable reduction in chemical oxygen demand and accumulation of sulfate. Furthermore, bioaugmentation with sulfur-metabolizing consortia improved system stability, and the recycling of sulfur fillers significantly reduced operating costs compared to conventional heterotrophic processes. This work establishes SADN as a sustainable and cost-effective strategy for advanced tannery wastewater treatment, with future research directed at elucidating the impact of salinity on the functional microbial community. The revealed synergistic mixotrophic metabolism, where autotrophic denitrifiers and endogenous heterotrophs synergistically remove nitrogen and organic carbon, provides a carbon-efficient treatment strategy that eliminates the need for external carbon input.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Unraveling Successional Trajectories of Functional Microorganisms and Sulfur-Mediated Metabolic Pathways in Advanced Nitrogen Removal of Tannery Wastewater

  • 期刊论文

  • 发表日期：

    2025年11月17日

查看全文
  • Rui Fang,
  • Huixue Ren,
  • Ning Guo,
  • Yan Jin,
  • Weichun Liang,
  • Chen Wei,
  • Liya Wang,
  • Shubing Han

摘要

Tannery wastewater presents a significant challenge for biological treatment due to its high concentrations of nitrogen and sulfide. Conventional heterotrophic denitrification is often limited by the requirement for costly organic carbon supplementation. This study demonstrates efficient nitrogen removal from authentic tannery wastewater without external carbon input, achieved under synergistic mixotrophic conditions. Integrated multi-omics analyses revealed sulfur autotrophic denitrification (SADN) as the primary nitrogen removal pathway. Concurrently, endogenous heterotrophs (e.g., Bacteroidota) degraded inherent organic matter, contributing to chemical oxygen demand (COD) removal. By integrating multi-omics analyses (16S rRNA sequencing, metagenomics, and metaproteomics), we uncovered the underlying microbial synergy. The process was facilitated by a succession of functional microorganisms, predominantly Proteobacteria and Bacteroidota, which facilitated simultaneous sulfur oxidation and organic matter degradation. We identified a complete enzymatic electron transfer chain, coupling sulfur oxidation with denitrification, which was strongly supported by the co-expression of key relevant genes and the high abundance of their corresponding core enzymes. This microbial synergy resulted in a substantially enhanced total nitrogen removal rate without any organic carbon input, alongside a notable reduction in chemical oxygen demand and accumulation of sulfate. Furthermore, bioaugmentation with sulfur-metabolizing consortia improved system stability, and the recycling of sulfur fillers significantly reduced operating costs compared to conventional heterotrophic processes. This work establishes SADN as a sustainable and cost-effective strategy for advanced tannery wastewater treatment, with future research directed at elucidating the impact of salinity on the functional microbial community. The revealed synergistic mixotrophic metabolism, where autotrophic denitrifiers and endogenous heterotrophs synergistically remove nitrogen and organic carbon, provides a carbon-efficient treatment strategy that eliminates the need for external carbon input.