<p>This work evaluated the application of naturally available biopolymers as biodispersants to stabilize crude oil droplets instead of harmful synthetic chemical dispersants and enhance their degradation. The biopolymers xanthan gum (XG) and oligo-lactic acid conjugate chitosan (OCH) were used as biodispersants, an alternative to the chemical dispersants used in oil spill remediation. A considerably stable crude oil-in-water emulsion was obtained with the application of XG and OCH. Particle size analysis and optical microscopic imaging confirmed the stability of the emulsion for 20&#xa0;days. Furthermore, the stabilized emulsion droplets were degraded by the isolated bacterium <i>Pseudomonas aeruginosa</i> CoE-SusPol3, and the results obtained from GC analysis confirmed greater degradation. The degradation percentage obtained for the stabilized emulsion was 74.31%, whereas that for the unstabilized emulsion was 34.06%. The in situ production of biosurfactants from the isolated bacteria also assisted in the degradation of hydrocarbons present in the crude oil by decreasing the surface tension of the crude oil to 28.53 (± 0.05) from 32.44 (± 0.22). Thus, the results obtained indicated that the synergistic effect of biodispersants and biosurfactants enhanced crude oil degradation by <i>Pseudomonas aeruginosa</i>. Hence, the use of such biobased materials could be a potential and sustainable solution for the treatment of oil spills.</p> Graphical Abstract <p></p>