<p>Cadmium (Cd) contamination in soils poses a significant environmental threat. This study evaluated seven biodegradable chelating agents for their potential to activate Cd in soil and enhance phytoremediation using marigold. Based on Cd speciation in soil, plant biomass, chlorophyll content and Cd accumulation, glycine, aspartic acid, and cysteine were identified as the most effective chelators. These were further combined in different ratios, and the composite chelator GAC6 (glycine:aspartic acid:cysteine = 4:10:6) showed the greatest synergistic effect. GAC6 increased bioavailable Cd in soil by over 70%. Compared to the control, GAC6 increased plant biomass and chlorophyll content by 11.86% and 21.18%, respectively, while also promoting Cd accumulation in plant tissues. Mechanistic analysis indicated that GAC6 improved plant physiological status, including water content and height, alleviating Cd-induced stress. It also reduced malondialdehyde (MDA) levels (12.6 U/g) and increased superoxide dismutase (SOD) activity (245.8 U/g), suggesting enhanced antioxidant defense. Additionally, GAC6 improved the soil environment by stabilizing pH (6.23), maintaining electrical conductivity (177.86 μS/cm), and increasing organic matter (65.79&#xa0;g/kg), thereby promoting microbial activity and nutrient uptake. Overall, GAC6 significantly enhanced the phytoextraction capacity of marigold, providing a promising and friendly strategy for Cd-contaminated soil remediation.</p>

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Biodegradable Chelator Enhances Cadmium Phytoextraction in Marigold: Synergistic Effects and Mechanistic Insights

  • Jian Cao,
  • Chenyang Lv,
  • Yadong Zhang,
  • Haishen Jiang,
  • Chenxu Zhang,
  • Fengxiang Yin,
  • Yao Yu,
  • Lingxiao Gao

摘要

Cadmium (Cd) contamination in soils poses a significant environmental threat. This study evaluated seven biodegradable chelating agents for their potential to activate Cd in soil and enhance phytoremediation using marigold. Based on Cd speciation in soil, plant biomass, chlorophyll content and Cd accumulation, glycine, aspartic acid, and cysteine were identified as the most effective chelators. These were further combined in different ratios, and the composite chelator GAC6 (glycine:aspartic acid:cysteine = 4:10:6) showed the greatest synergistic effect. GAC6 increased bioavailable Cd in soil by over 70%. Compared to the control, GAC6 increased plant biomass and chlorophyll content by 11.86% and 21.18%, respectively, while also promoting Cd accumulation in plant tissues. Mechanistic analysis indicated that GAC6 improved plant physiological status, including water content and height, alleviating Cd-induced stress. It also reduced malondialdehyde (MDA) levels (12.6 U/g) and increased superoxide dismutase (SOD) activity (245.8 U/g), suggesting enhanced antioxidant defense. Additionally, GAC6 improved the soil environment by stabilizing pH (6.23), maintaining electrical conductivity (177.86 μS/cm), and increasing organic matter (65.79 g/kg), thereby promoting microbial activity and nutrient uptake. Overall, GAC6 significantly enhanced the phytoextraction capacity of marigold, providing a promising and friendly strategy for Cd-contaminated soil remediation.