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<p>Bio-stimulants derived from agro-industrial waste through the addition of probiotic provide specific conditions that enhance soil quality by increasing nutrient levels, organic matter, and microbial activity, thereby optimizing nutrient availability and functionality. In this study, biotransformation was evaluated on a 20 L pilot scale with a usable volume of 15 L using seed flasks with a 10% v/v inoculum (<i>L. casei</i>) and pig manure supplemented with whey (PMSW) (90% v/v), and pH (7). PMSW was evaluated in terms of: TS (3.48%), TSV (75.83%), carbohydrates, total nitrogen (0.52%), protein (2.84%), fecal coliforms and <i>Salmonella</i> (&lt; 1000). The transformed biomasses were applied into the roots of <i>Pinus pseudostrobus</i> and <i>Cupressus</i> sp<i>.</i>. Were obtained from the community nursery and allowed to grow for 180&#xa0;days. The height, diameter, and chlorophyll content of the trees were evaluated. The application of the bio-stimulant had an effect on the growth and morphological characteristics of the species, which can be attributed to the addition of microorganisms and the availability of nutrients. <i>P. pseudostrobus</i> seedlings were obtained with a height of 15.6&#xa0;cm and a diameter of 56&#xa0;mm, while <i>cupressus</i> seedlings had a height of 56&#xa0;cm and a diameter of 65&#xa0;mm, this being the one that obtained the best results. Therefore, this type of bio-stimulant represents a viable option for use in forest soils, given that it allows the production of quality seedlings and promotes the circular economy of native peoples.</p>

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Valorization of Agro-industrial Waste to Produce a Probiotic-bio-stimulant Through the Anaerobic Co-fermentation Process With Lactobacillus Casei: a Circular Economy Approach in Vulnerable Communities of Mexico

  • 期刊论文

  • 发表日期：

    2025年10月4日

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  • Viviana Rodríguez-Rivera,
  • Joaquín Estrada-García,
  • Roger Emmanuel Sales-Pérez,
  • José Manuel Hernández-Martínez,
  • Juan Manuel Méndez-Contreras

摘要

Bio-stimulants derived from agro-industrial waste through the addition of probiotic provide specific conditions that enhance soil quality by increasing nutrient levels, organic matter, and microbial activity, thereby optimizing nutrient availability and functionality. In this study, biotransformation was evaluated on a 20 L pilot scale with a usable volume of 15 L using seed flasks with a 10% v/v inoculum (L. casei) and pig manure supplemented with whey (PMSW) (90% v/v), and pH (7). PMSW was evaluated in terms of: TS (3.48%), TSV (75.83%), carbohydrates, total nitrogen (0.52%), protein (2.84%), fecal coliforms and Salmonella (< 1000). The transformed biomasses were applied into the roots of Pinus pseudostrobus and Cupressus sp.. Were obtained from the community nursery and allowed to grow for 180 days. The height, diameter, and chlorophyll content of the trees were evaluated. The application of the bio-stimulant had an effect on the growth and morphological characteristics of the species, which can be attributed to the addition of microorganisms and the availability of nutrients. P. pseudostrobus seedlings were obtained with a height of 15.6 cm and a diameter of 56 mm, while cupressus seedlings had a height of 56 cm and a diameter of 65 mm, this being the one that obtained the best results. Therefore, this type of bio-stimulant represents a viable option for use in forest soils, given that it allows the production of quality seedlings and promotes the circular economy of native peoples.