<p>Over the past few decades, numerous researchers have focused on creating novel, practical, affordable, and easy-to-use techniques for determining and estimating organic pollutants in aquatic environments. This study aims to determine ADP drug using KMnO₄ as reagent by spectrofluorometric method. At pH 4, the fluorescence spectrum of the produced complex ion association [Mn (II)-ADP] was obtained at 420 nm with high intensity. The quantification and detection limits were 0.56 and 0.18 µg mL⁻¹, respectively. The Stern–Volmer and the apparent binding constant were calculated to be 158.6 and 159.95 L mol⁻¹, respectively. Also, different variables were investigated such as KMnO₄ concentration, and the optimized conditions were pH 4, and 0.1 µg mL⁻¹, respectively. In studied samples, adapalene recovery percentages ranged from (90–95%), and (90–93%) in wastewater, and sea water, respectively. These results represent an exceptional and a perfect recovery percentage.</p>