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<p>Complete genome sequences were determined for three uncharacterized betabaculoviruses (<i>Baculoviridae</i>: <i>Lefavirales</i>) from noctuid moth species. These viruses included Eudryas unio granulovirus 1112 (EuunGV-1112), Eudryas unio granulovirus 1229 (EuunGV-1229), and Autographa gamma granulovirus&#xa0;Darmstadt (AugaGV-Darmstadt). Analysis of the features and open reading frame (ORF) contents of the genome sequences indicated that all three viruses were closely related to Helicoverpa armigera granulovirus (HearGV; <i>Betabaculovirus helarmigerae</i>) and Xestia c-nigrum granulovirus (XecnGV; <i>Betabaculovirus xecnigri</i>), two betabaculoviruses that kill larvae slowly and appear to suppress alphabaculovirus replication in coinfections. Phylogenetic inference with whole genome or core gene alignments grouped the EuunGV isolates in a clade with XecnGV. This clade was contained within a larger clade with AugaGV. Pairwise nucleotide distance estimation confirmed that AugaGV and the EuunGV isolates are viruses of <i>Betabaculovirus xecnigri</i>. Five ORFs were found to be unique to the EuunGV/AugaGV/HearGV/XecnGV group of betabaculoviruses, though what role the products of these ORFs play in the unusual features of their pathology remains to be determined. PCR analysis of cadavers from bioassays of AugaGV and EuunGV indicated that these viruses are able to infect and replicate in larvae of <i>Helicoverpa zea</i> and <i>Spodoptera frugiperda</i>, species which are susceptible to HearGV.</p>

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Genome sequence analysis of baculoviruses from the noctuid moths Eudryas unio and Autographa gamma indicates that they are isolates of Betabaculovirus xecnigri, a species of slow-killing granuloviruses

  • 期刊论文

  • 发表日期：

    2026年7月22日

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  • Robert L. Harrison,
  • Charlotte B. Francoeur,
  • Daniel L. Rowley

摘要

Complete genome sequences were determined for three uncharacterized betabaculoviruses (Baculoviridae: Lefavirales) from noctuid moth species. These viruses included Eudryas unio granulovirus 1112 (EuunGV-1112), Eudryas unio granulovirus 1229 (EuunGV-1229), and Autographa gamma granulovirus Darmstadt (AugaGV-Darmstadt). Analysis of the features and open reading frame (ORF) contents of the genome sequences indicated that all three viruses were closely related to Helicoverpa armigera granulovirus (HearGV; Betabaculovirus helarmigerae) and Xestia c-nigrum granulovirus (XecnGV; Betabaculovirus xecnigri), two betabaculoviruses that kill larvae slowly and appear to suppress alphabaculovirus replication in coinfections. Phylogenetic inference with whole genome or core gene alignments grouped the EuunGV isolates in a clade with XecnGV. This clade was contained within a larger clade with AugaGV. Pairwise nucleotide distance estimation confirmed that AugaGV and the EuunGV isolates are viruses of Betabaculovirus xecnigri. Five ORFs were found to be unique to the EuunGV/AugaGV/HearGV/XecnGV group of betabaculoviruses, though what role the products of these ORFs play in the unusual features of their pathology remains to be determined. PCR analysis of cadavers from bioassays of AugaGV and EuunGV indicated that these viruses are able to infect and replicate in larvae of Helicoverpa zea and Spodoptera frugiperda, species which are susceptible to HearGV.