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<p>Vector-borne diseases in dogs are an emerging problem worldwide due to their frequency, morbidity, and zoonotic relevance. Since there are no previous studies determining the frequency of <i>Ehrlichia canis</i> and <i>Rickettsia rickettsii</i> infection in three neighborhoods of the city of Chihuahua, Mexico, using molecular detection techniques, the objective of the present study was to detect, by nested polymerase chain reaction (PCR), a fragment of the <i>gp36</i> gene of <i>E. canis</i> and the <i>ompA</i> gene of <i>R. rickettsii</i> in tick-infested dogs captured at the canine control center of the city of Chihuahua, and to phylogenetically analyze the genotypes present. Blood samples were collected from 123 tick-infested dogs. DNA was extracted from peripheral blood leukocytes and used in nested PCR protocols. The results showed a positivity frequency of 16.2% (20/123) for <i>E. canis</i> and 3.2% (4/123) for <i>R. rickettsii</i>. Six sequenced samples corresponded to <i>E. canis</i> and four to <i>R. rickettsii</i>. Phylogenetic analysis showed a close relationship with sequences previously described in the United States. This is the first molecular identification confirming <i>E. canis</i> and <i>R. rickettsii</i> infection in tick-infested dogs from some neighborhoods of the Chihuahua city, Mexico.</p>

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Molecular detection of Ehrlichia canis and Rickettsia rickettsii in tick-infested dogs from the city of Chihuahua, Mexico: phylogenetic analysis of the gp36 and ompA genes

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年8月1日

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  • Marcos Javier Sánchez Pérez,
  • Guadalupe Gordillo-Pérez,
  • Cecilia Rodríguez-Murillo,
  • Susana Elvira García Vázquez,
  • Anaid Ireri Hernández García,
  • Gabriel Eduardo Acevedo-Jiménez,
  • Hugo Ramírez Álvarez

摘要

Vector-borne diseases in dogs are an emerging problem worldwide due to their frequency, morbidity, and zoonotic relevance. Since there are no previous studies determining the frequency of Ehrlichia canis and Rickettsia rickettsii infection in three neighborhoods of the city of Chihuahua, Mexico, using molecular detection techniques, the objective of the present study was to detect, by nested polymerase chain reaction (PCR), a fragment of the gp36 gene of E. canis and the ompA gene of R. rickettsii in tick-infested dogs captured at the canine control center of the city of Chihuahua, and to phylogenetically analyze the genotypes present. Blood samples were collected from 123 tick-infested dogs. DNA was extracted from peripheral blood leukocytes and used in nested PCR protocols. The results showed a positivity frequency of 16.2% (20/123) for E. canis and 3.2% (4/123) for R. rickettsii. Six sequenced samples corresponded to E. canis and four to R. rickettsii. Phylogenetic analysis showed a close relationship with sequences previously described in the United States. This is the first molecular identification confirming E. canis and R. rickettsii infection in tick-infested dogs from some neighborhoods of the Chihuahua city, Mexico.