Scholarlink

<p>The health of dairy goat herds may be compromised by infections caused by small ruminant lentiviruses (SRLV) and <i>Mycoplasma agalactiae</i>, particularly under coinfection conditions. This study investigated a dairy goat herd composed of 38 animals with a history of joint enlargement and reduced milk production in Northeastern Brazil. Clinical, serological, molecular, and phylogenetic analyses were performed using blood, milk, synovial fluid, and ocular swab samples. Clinically, arthritis was observed in 28.9% (11/38) of the animals, keratoconjunctivitis in 28.9% (11/38), and mastitis or agalactia in 19.4% (6/31) of the lactating females. For SRLV diagnosis, positivity rates were 31.6% (12/38) by agar gel immunodiffusion (AGID) and 65.8% (25/38) by indirect ELISA, with moderate agreement between tests (K = 0.387; <i>P</i> = 0.002). Indirect ELISA for <i>Mycoplasma</i> spp. detected antibodies in 34.2% (13/38) of the animals, while PCR identified <i>M. agalactiae</i> in 83.3% (5/6) of synovial fluid samples, 66.7% (2/3) of ocular swabs, and 79.3% (23/29) of milk samples. Concomitant clinical manifestations were observed exclusively in coinfected animals. Phylogenetic analysis based on the 16&#xa0;S rRNA gene supported the molecular identification of <i>M. agalactiae</i>. These findings demonstrate the concomitant circulation of SRLV and <i>M. agalactiae</i> in the investigated herd and reinforce the importance of combining clinical evaluation with serological and molecular methods applied to different biological samples for the diagnosis of coinfections in goats.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Concomitant circulation of lentiviruses and Mycoplasma spp. in dairy goats: a clinical and multi-sample diagnostic approach

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月24日

查看全文
  • Huber Rizzo,
  • Natália Carrillo Gaeta,
  • Jeferson Silva Carvalho,
  • Felipe Gabriel Carneiro Pessoa,
  • Jerônimo Hugo de Souza,
  • Guilherme José da Costa e Silva,
  • Ana Claudia Campos,
  • Edísio Oliveira de Azevedo,
  • Lilian Gregory,
  • Lúcio Esmeraldo Honório de Melo

摘要

The health of dairy goat herds may be compromised by infections caused by small ruminant lentiviruses (SRLV) and Mycoplasma agalactiae, particularly under coinfection conditions. This study investigated a dairy goat herd composed of 38 animals with a history of joint enlargement and reduced milk production in Northeastern Brazil. Clinical, serological, molecular, and phylogenetic analyses were performed using blood, milk, synovial fluid, and ocular swab samples. Clinically, arthritis was observed in 28.9% (11/38) of the animals, keratoconjunctivitis in 28.9% (11/38), and mastitis or agalactia in 19.4% (6/31) of the lactating females. For SRLV diagnosis, positivity rates were 31.6% (12/38) by agar gel immunodiffusion (AGID) and 65.8% (25/38) by indirect ELISA, with moderate agreement between tests (K = 0.387; P = 0.002). Indirect ELISA for Mycoplasma spp. detected antibodies in 34.2% (13/38) of the animals, while PCR identified M. agalactiae in 83.3% (5/6) of synovial fluid samples, 66.7% (2/3) of ocular swabs, and 79.3% (23/29) of milk samples. Concomitant clinical manifestations were observed exclusively in coinfected animals. Phylogenetic analysis based on the 16 S rRNA gene supported the molecular identification of M. agalactiae. These findings demonstrate the concomitant circulation of SRLV and M. agalactiae in the investigated herd and reinforce the importance of combining clinical evaluation with serological and molecular methods applied to different biological samples for the diagnosis of coinfections in goats.