Scholarlink

<p>Passive surveillance of fallen wild boar represents an important tool for detecting notifiable diseases such as Classical swine fever (CSF) and African swine fever (ASF) which are showing comparable pathological findings. For differential diagnosis, it is advantageous to use one sample matrix for testing of both diseases. Especially, if no serum sample can be collected from wild suids, blood swabs represent an alternative matrix in ASF diagnosis. On this basis, the present study aimed to analyze blood swabs serologically using CSF antibody ELISAs and virus neutralization tests. In total, twenty-nine CSF antibody positive samples and one-hundred CSF antibody negative samples were examined. The results show that this sample matrix is also suitable for detecting antibodies against CSF virus (CSFV). In general, the sensitivity of blood swabs is slightly reduced compared to the analysis of serum, which can affect the classification of samples taken during early seroconversion. However, samples collected at  ≥ 21 days post CSFV infection are tested safely antibody positive.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Blood swabs represent an alternative sample matrix for detection of antibodies against classical swine fever virus during surveillance in wild boar

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月25日

查看全文
  • Denise Meyer,
  • Sandra Blome,
  • Lia Ebner,
  • Paul Becher

摘要

Passive surveillance of fallen wild boar represents an important tool for detecting notifiable diseases such as Classical swine fever (CSF) and African swine fever (ASF) which are showing comparable pathological findings. For differential diagnosis, it is advantageous to use one sample matrix for testing of both diseases. Especially, if no serum sample can be collected from wild suids, blood swabs represent an alternative matrix in ASF diagnosis. On this basis, the present study aimed to analyze blood swabs serologically using CSF antibody ELISAs and virus neutralization tests. In total, twenty-nine CSF antibody positive samples and one-hundred CSF antibody negative samples were examined. The results show that this sample matrix is also suitable for detecting antibodies against CSF virus (CSFV). In general, the sensitivity of blood swabs is slightly reduced compared to the analysis of serum, which can affect the classification of samples taken during early seroconversion. However, samples collected at  ≥ 21 days post CSFV infection are tested safely antibody positive.