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<p>This study documents the first confirmed case of JSRV-associated nasal adenocarcinoma in a goat through a comprehensive analysis combining histopathological, immunohistochemical, and molecular techniques.</p><p>An autopsy was conducted on a 12-month-old goat exhibiting progressive nasal obstruction. Tissue samples from the nasal mass were examined histologically using H&amp;E staining. Immunohistochemical (IHC) analysis was performed to detect JSRV capsid protein expression and to assess cellular proliferation markers (Ki-67 and PCNA). Molecular screening via PCR was carried out using primers specific for JSRV, ENTV-1, ENTV-2. The JSRV-positive PCR amplicon was sequenced and subjected to phylogenetic analysis.</p><p>PCR analysis confirmed the presence of JSRV proviral DNA (385&#xa0;bp) in the tumor tissue, while results for ENTV-1 and ENTV-2 were negative. Histopathological examination identified a mixed glandular adenocarcinoma arising from the surface and glandular epithelium, with evidence of turbinate bone invasion. IHC revealed multifocal positivity for JSRV capsid antigen and indicated a high proliferation index (30–35%). Phylogenetic analysis classified the detected viral strain within the exogenous JSRV group. The infected goat originated from a herd with no documented contact with sheep.</p><p>This case provides the first conclusive evidence of JSRV-induced nasal adenocarcinoma in a goat, indicating a potential expansion of the virus’s host and tissue tropism. These findings highlight the necessity for further investigation into the viral envelope-receptor interactions and promoter elements that govern JSRV tropism.</p>

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Nasal adenocarcinoma induced by jaagsiekte sheep retrovirus in a juvenile goat: first report on pathological and molecular findings

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月13日

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  • Yesari Eroksuz,
  • Mehmet Ozkan Timurkan,
  • Engin Berber,
  • Burak Karabulut,
  • Emel Kara,
  • Hayrunnisa Bostan-Yoru,
  • Hatice Eroksuz

摘要

This study documents the first confirmed case of JSRV-associated nasal adenocarcinoma in a goat through a comprehensive analysis combining histopathological, immunohistochemical, and molecular techniques.

An autopsy was conducted on a 12-month-old goat exhibiting progressive nasal obstruction. Tissue samples from the nasal mass were examined histologically using H&E staining. Immunohistochemical (IHC) analysis was performed to detect JSRV capsid protein expression and to assess cellular proliferation markers (Ki-67 and PCNA). Molecular screening via PCR was carried out using primers specific for JSRV, ENTV-1, ENTV-2. The JSRV-positive PCR amplicon was sequenced and subjected to phylogenetic analysis.

PCR analysis confirmed the presence of JSRV proviral DNA (385 bp) in the tumor tissue, while results for ENTV-1 and ENTV-2 were negative. Histopathological examination identified a mixed glandular adenocarcinoma arising from the surface and glandular epithelium, with evidence of turbinate bone invasion. IHC revealed multifocal positivity for JSRV capsid antigen and indicated a high proliferation index (30–35%). Phylogenetic analysis classified the detected viral strain within the exogenous JSRV group. The infected goat originated from a herd with no documented contact with sheep.

This case provides the first conclusive evidence of JSRV-induced nasal adenocarcinoma in a goat, indicating a potential expansion of the virus’s host and tissue tropism. These findings highlight the necessity for further investigation into the viral envelope-receptor interactions and promoter elements that govern JSRV tropism.