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<p>The aim was to study the effect of long-acting analogue of oxytocin (Carbetocin) on cervical collagenolysis of MAP-eCG synchronized ewes. At the expected time of artificial insemination, five ewes were slaughtered (<i>n</i> = 5) and their cervical explants (100–200&#xa0;mg) were incubated during 12&#xa0;h with MEM supplemented with 0, 8, 16, 32 and 64 ng/mL of Cb. Activities of activated and latent forms of matrix metalloproteinases-2 and − 9 (MMP-2 and MMP-9, respectively) in the supernatant were determined by a SDS-PAGE zymography and prostaglandin E2 concentration by immunoassay. Data were analyzed by ANOVA test. The MMP-9 activity was detected weakly and sporadically. The activity of activated MMP-2 was higher in 32 ng/mL Carbetocin treated samples than in untreated samples (<i>P</i> &lt; 0.03). The activity of latent MMP-2 tended to be higher in 32 ng/mL than in 8 ng/mL Carbetocin treated samples (<i>P</i> = 0.083). The activated/latent ratio of MMP-2 tended to be higher in 64 ng/mL Cb treated samples than in untreated samples (<i>P</i> = 0.089). Prostagladin E2 concentration was not affected by Carbetocin dose. The data show that Carbetocin treatment of cervical explants induces an increase in MMP-2 activity by prostaglandin E2-independent mechanisms, suggesting Carbetocin as a potential inducer of cervical dilation in sheep.</p>

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Increasing matrix metalloproteinase-2 activity by treatment of ovine cervical explants with a long-acting analogue of oxytocin (Carbetocin) at the expected time of artificial insemination

  • 期刊论文

  • 发表日期：

    2025年1月22日

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  • Marcelo Rodríguez-Piñón,
  • Gonzalo García-Barcelo

摘要

The aim was to study the effect of long-acting analogue of oxytocin (Carbetocin) on cervical collagenolysis of MAP-eCG synchronized ewes. At the expected time of artificial insemination, five ewes were slaughtered (n = 5) and their cervical explants (100–200 mg) were incubated during 12 h with MEM supplemented with 0, 8, 16, 32 and 64 ng/mL of Cb. Activities of activated and latent forms of matrix metalloproteinases-2 and − 9 (MMP-2 and MMP-9, respectively) in the supernatant were determined by a SDS-PAGE zymography and prostaglandin E2 concentration by immunoassay. Data were analyzed by ANOVA test. The MMP-9 activity was detected weakly and sporadically. The activity of activated MMP-2 was higher in 32 ng/mL Carbetocin treated samples than in untreated samples (P < 0.03). The activity of latent MMP-2 tended to be higher in 32 ng/mL than in 8 ng/mL Carbetocin treated samples (P = 0.083). The activated/latent ratio of MMP-2 tended to be higher in 64 ng/mL Cb treated samples than in untreated samples (P = 0.089). Prostagladin E2 concentration was not affected by Carbetocin dose. The data show that Carbetocin treatment of cervical explants induces an increase in MMP-2 activity by prostaglandin E2-independent mechanisms, suggesting Carbetocin as a potential inducer of cervical dilation in sheep.