<p>Urbanization is a major driver of biodiversity loss, particularly affecting pollinators, which are crucial for ecosystem functioning and food production. Pollinator habitat in fragmented urban landscapes can support diverse bee communities, however, understanding local and landscape factors mediating pollinator ability to utilize floral resources will aid conservation actions. This study investigates how varying land-use intensity and floral resource availability influence pollinator abundance, community composition, and functional trait diversity across a gradient of urban development in central Arkansas. Using a combination of hand-netting and bee bowls, we collected a total of 1,262 pollinators from 151 species across 12 study sites. Our results showed no significant difference in overall pollinator abundance and species richness among land-use categories. However, butterfly abundance was significantly highest in areas with low disturbance, and honeybee abundance was significantly highest in areas with high disturbance. Pollinator beta diversity was driven mainly by species turnover, indicating strong community restructuring with urbanization. In contrast with our hypothesis, functional diversity was significantly higher in study sites in areas with high and moderate levels of disturbance, influenced mostly by nesting traits. Ground-nesting bees declined in areas with high disturbance, while polylectic bees and corbiculate bees were more prevalent in high disturbance sites. These findings highlight the role of functional traits, particularly nesting preferences, in mediating pollinator responses to urbanization and emphasize the importance of managed green spaces for conserving diverse pollinator communities in urban landscapes.</p>