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<p>The first experiment assessed the effect of bovine herpesvirus 1 (BoHV-1) on thawed bull semen. Semen samples testing negative for BoHV-1 via Nested-PCR were incubated at 37&#xa0;°C with viral concentrations of 0 (control), 10⁴, 10⁵, and 10⁶ TCID₅₀/mL. Sperm quality parameters were evaluated over an 8-hour period. The second experiment assessed the efficacy of two protocols for reducing the viral load (viral titration) in experimentally infected semen (10⁴ TCID₅₀/mL): Percoll gradient (PG) and magnetic nanoparticles coupled with anti-BoHV-1 antibodies (MNPs). Sperm kinematics were influenced by both bull and time, with bull × time interaction (<i>P</i> &lt; 0.001). Membrane integrity and morphology were influenced only by time (<i>P</i> &lt; 0.01), with no effect of viral infection (<i>P</i> &gt; 0.05). In viral titration, all PG-treated samples tested negative. Samples treated with MNPs also yielded negative results after a 60&#xa0;min-incubation. It is concluded that BoHV-1 had no direct effect on thawed bull sperm quality and that PG appears to be effective removing the viral load. Furthermore, MNP is a viable alternative for virus control in semen, with no apparent deleterious effects to sperm motility.</p>

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Effects of bovine herpesvirus 1 on thawed bull sperm and evaluation of methods for its removal from experimentally infected semen

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月24日

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  • Ivana Ferro Carmo,
  • Emelly Barbosa Calheiros,
  • Juliana Carla Cavalcanti Marques,
  • Lucas Santos Matos,
  • Abelardo Silva-Júnior,
  • Diogo Ribeiro Câmara

摘要

The first experiment assessed the effect of bovine herpesvirus 1 (BoHV-1) on thawed bull semen. Semen samples testing negative for BoHV-1 via Nested-PCR were incubated at 37 °C with viral concentrations of 0 (control), 10⁴, 10⁵, and 10⁶ TCID₅₀/mL. Sperm quality parameters were evaluated over an 8-hour period. The second experiment assessed the efficacy of two protocols for reducing the viral load (viral titration) in experimentally infected semen (10⁴ TCID₅₀/mL): Percoll gradient (PG) and magnetic nanoparticles coupled with anti-BoHV-1 antibodies (MNPs). Sperm kinematics were influenced by both bull and time, with bull × time interaction (P < 0.001). Membrane integrity and morphology were influenced only by time (P < 0.01), with no effect of viral infection (P > 0.05). In viral titration, all PG-treated samples tested negative. Samples treated with MNPs also yielded negative results after a 60 min-incubation. It is concluded that BoHV-1 had no direct effect on thawed bull sperm quality and that PG appears to be effective removing the viral load. Furthermore, MNP is a viable alternative for virus control in semen, with no apparent deleterious effects to sperm motility.