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<p>The present study describes the prevalence of different strains of Contagious ecthyma virus in Tamil Nadu by nucleotide sequencing and phylogenetic analysis of full length A32L gene. Molecular characterization and multiple nucleotide alignment of A32L gene of the four CEV isolates exposed that the nucleotide and deduced amino acid sequences of CEV isolates had 96.8–99.2 and 96–99% similarity with other compared isolates and mutations/deletions at 12, 7, 5 and 17 positions in Namakkal, Salem, Dharmapuri and Krishnagiri isolates respectively. Furthermore, the phylogenetic analysis indicated Namakkal, Salem and Krishnagiri isolates were closely related to Bangalore and Mukteswar 59/05, India isolates while the Dharmapuri isolate with Assam, Fujian GO and SJ1 strain. The deduced amino acid sequences of CEV ATPase analyzed for five functional motifs in the N-terminal (I, II, III, IV and V) and KGD along with RGD motifs in the C-terminal revealed a single amino acid substitution in motif V of Namakkal isolate and three amino acid substitutions in motif III of Krishnagiri isolate besides the presence of two KGD repeats at position 247–252 and two RGD motifs at position 256–258 and 267–269 in all the four CEV isolates. The predicted secondary structure of ATPase confirmed the presence of conserved arginine finger residue at position 125 in motif III and structural similarity with genome packaging NTPases of other viruses. The A32L gene based molecular analysis elucidate the occurrence of non-identical strains of CEV in the study area and dependable for virus characterization.</p>

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Molecular characterization of Indian contagious ecthyma (Orf) virus isolates by A32L gene and its encoded ATPase protein

  • 期刊论文

  • 发表日期：

    2026年2月2日

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  • Sowmiya P. V.,
  • Ramya Kalaivanan,
  • Sukumar Kuppannan,
  • Madheswaran Rajamanickam,
  • Ponnusamy Periyasamy,
  • Selvaraju Mani

摘要

The present study describes the prevalence of different strains of Contagious ecthyma virus in Tamil Nadu by nucleotide sequencing and phylogenetic analysis of full length A32L gene. Molecular characterization and multiple nucleotide alignment of A32L gene of the four CEV isolates exposed that the nucleotide and deduced amino acid sequences of CEV isolates had 96.8–99.2 and 96–99% similarity with other compared isolates and mutations/deletions at 12, 7, 5 and 17 positions in Namakkal, Salem, Dharmapuri and Krishnagiri isolates respectively. Furthermore, the phylogenetic analysis indicated Namakkal, Salem and Krishnagiri isolates were closely related to Bangalore and Mukteswar 59/05, India isolates while the Dharmapuri isolate with Assam, Fujian GO and SJ1 strain. The deduced amino acid sequences of CEV ATPase analyzed for five functional motifs in the N-terminal (I, II, III, IV and V) and KGD along with RGD motifs in the C-terminal revealed a single amino acid substitution in motif V of Namakkal isolate and three amino acid substitutions in motif III of Krishnagiri isolate besides the presence of two KGD repeats at position 247–252 and two RGD motifs at position 256–258 and 267–269 in all the four CEV isolates. The predicted secondary structure of ATPase confirmed the presence of conserved arginine finger residue at position 125 in motif III and structural similarity with genome packaging NTPases of other viruses. The A32L gene based molecular analysis elucidate the occurrence of non-identical strains of CEV in the study area and dependable for virus characterization.