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<p>Growth traits are critical economic factors in sheep breeding and are influenced by various elements. This study aims to identify the expression levels of the <i>LGALS3</i> and <i>XPNPEP2</i> genes in Hu sheep, as well as to explore the association between single nucleotide polymorphisms (SNPs) and growth traits using qRT-PCR, DNA sequencing, and AQP™ typing technology, thereby providing a theoretical basis for marker-assisted selection in sheep breeding. The results showed that both <i>LGALS3</i> and <i>XPNPEP2</i> genes had intronic mutations: g.65,688,711 T &gt; C and g.113,699,247&#xa0;C &gt; T. Both genes were expressed in all ten tissues analyzed, with <i>LGALS3</i> being most highly expressed in spleen tissue and <i>XPNPEP2</i> being most highly expressed in kidney tissue. Association analyses revealed that the <i>LGALS3</i> g.65,688,711 T &gt; C and <i>XPNPEP2</i> g.113,699,247&#xa0;C &gt; T polymorphisms were significantly associated with growth traits. Furthermore, analysis of different genotype combinations indicated that various combinations of <i>LGALS3</i> and <i>XPNPEP2</i> genotypes displayed significant differences in the growth traits of Hu sheep across different growth stages (<i>P</i> &lt; 0.05). Thus, the polymorphisms of the <i>LGALS3</i> and <i>XPNPEP2</i> genes may serve as potential markers for growth traits in Hu sheep.</p>

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Expression of LGALS3 and XPNPEP2 genes and their association with growth traits in sheep

  • 期刊论文

  • 发表日期：

    2025年10月13日

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  • Guoxing Jia,
  • Weimin Wang,
  • Huibing Tian,
  • Xiaojuan Wang,
  • Yukun Zhang,
  • Yuan Zhao,
  • Lijuan He,
  • Zongwu Ma,
  • Xioabing Yang,
  • Dan Xu,
  • Kunchao Han,
  • Peiliang Cao,
  • Mengru Pu,
  • Lingting Li,
  • Dewen Kong,
  • Jian Zhang,
  • Hongjian Li,
  • Qi Zhang,
  • Chenqi Yan,
  • Ziyue Xiao,
  • Lianjun Fen,
  • Bo Ma,
  • Xinji Wang,
  • Zhaocai Zhang,
  • Xiaoxue Zhang

摘要

Growth traits are critical economic factors in sheep breeding and are influenced by various elements. This study aims to identify the expression levels of the LGALS3 and XPNPEP2 genes in Hu sheep, as well as to explore the association between single nucleotide polymorphisms (SNPs) and growth traits using qRT-PCR, DNA sequencing, and AQP™ typing technology, thereby providing a theoretical basis for marker-assisted selection in sheep breeding. The results showed that both LGALS3 and XPNPEP2 genes had intronic mutations: g.65,688,711 T > C and g.113,699,247 C > T. Both genes were expressed in all ten tissues analyzed, with LGALS3 being most highly expressed in spleen tissue and XPNPEP2 being most highly expressed in kidney tissue. Association analyses revealed that the LGALS3 g.65,688,711 T > C and XPNPEP2 g.113,699,247 C > T polymorphisms were significantly associated with growth traits. Furthermore, analysis of different genotype combinations indicated that various combinations of LGALS3 and XPNPEP2 genotypes displayed significant differences in the growth traits of Hu sheep across different growth stages (P < 0.05). Thus, the polymorphisms of the LGALS3 and XPNPEP2 genes may serve as potential markers for growth traits in Hu sheep.