<p>This study evaluated the impact of aflatoxin B₁ (AFB₁) and ochratoxin A (OTA) on growth performance, organ health, lipid profiles, and immunity in broiler chickens. It also assessed the potential of dietary additives, a commercial toxin binder (1000&#xa0;mg/kg), yeast cell wall (YCW; 1000&#xa0;mg/kg), <i>Lactobacillus fermentum</i> (LF; 1000&#xa0;mg/kg, a total bacterial count of 1 × 10⁹ CFU/kg), and milk thistle extract (MTE; 200&#xa0;mg/kg), to mitigate the harmful effects of AFB₁ and OTA. A total of 280 one-day-old broiler chicks were assigned to seven treatments: negative control (NC), positive control (PC, fed a diet contaminated with AFB₁ and OTA), PC + a commercial toxin binder, PC + YCW, PC + LF, PC + MTE, and PC + (YCW + LF + MTE). The experiment lasted for 42 days. Results indicated that AFB₁ and OTA significantly reduced growth performance parameters, increased liver and kidney biomarkers, such as serum aspartate aminotransferase, alanine aminotransferase, and creatinine levels (<i>P</i> &lt; 0.05), indicative of organ damage. Blood lipid profiles were also adversely affected, as demonstrated by increased serum low-density lipoprotein, and decreased high-density lipoprotein levels (<i>P</i> &lt; 0.05). Immune function was compromised by AFB₁ and OTA, as indicated by lower serum total protein and globulin concentrations, and relative weights of Bursa of Fabricius (<i>P</i> &lt; 0.05). Dietary supplementation with YCW, LF, and MTE effectively mitigated these adverse effects, improving growth performance, organ health, lipid profiles, and immune function in broilers exposed to AFB₁ and OTA. In conclusion, this study highlights the potential of LF, YCW, and MTE as dietary supplements to counteract the harmful effects of AFB₁ and OTA in broiler chickens, suggesting their use as natural alternatives for mycotoxin mitigation in poultry production.</p>