<p>Non-enzymatic glucose sensors with promising electrochemical activity, high sensitivity, selectivity and stability are booming in a variety of fields. Herein, we have successfully synthesized the Co and Co/Zn-MOF materials and evaluated for the glucose sensing application. The successful preparation of Co and Co/Zn-MOF were confirmed by using the XRD, XPS, SEM and FT-IR characterizations. The detailed N₂ adsorption-desorption analysis has revealed the higher surface area, pore size and volume for the Co/Zn-MOF compared to Co-MOF. Remarkably, the Co/Zn-MOF@rGO showed higher sensing ability with a limit of detection (LOD) of 0.67 µM compared to that of the Co-MOF with an LOD of 1.65 µM. The lowest LOD of Co/Zn MOF@rGO might be due to the enhanced surface area and charge transportation. The as-prepared Co/Zn MOF@rGO has exhibited superior electrocatalytic glucose-sensing ability with remarkable stability and selectivity. Finally, the developed sensor has been tested for quantification of glucose from real sweat samples and results were obtained with good recoveries. The rGo-Co/Zn MOF hybrid composite, developed as a highly selective and sensitive non-enzymatic electrochemical glucose sensor, holds promise for continuous metabolic monitoring in disability research, where glucose regulation critically impacts health and rehabilitation outcomes.</p>