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<p>In this work, ZnO (zinc oxide) nanostructures were electrodeposited onto a glassy carbon electrode (GCE) using a simple, environmentally friendly, and low-cost electrochemical method. The electrocatalytic oxidation of uric acid (UA) was investigated at this ZnO/GCE. Electrochemical oxidation of uric acid in 0.1&#xa0;M phosphate buffer solution PBS (0.1M K<sub>2</sub>HPO<sub>4</sub> + 0.1&#xa0;M KH<sub>2</sub>PO<sub>4</sub>) at pH = 7 was examined by cyclic voltammetry. The formation and structure of the ZnO/GCE were systematically characterized by SEM, EDX and XRD. These analyses showed that the synthetized ZnO nanostructures possessed good-crystallinity with well-defined nanorod morphology and favorable characteristics. The electrochemical response of the as-prepared ZnO-based sensor toward uric acid in PBS was further evaluated by linear sweep voltammetry (LSV) and chronoamperometry. The results confirmed that ZnO-modified GCE exhibited high electrocatalytic activity toward uric acid oxidation. Furthermore, the sensor demonstrated a high sensitivity of 28.4 µA µM<sup>−1</sup> cm<sup>−2</sup> and a low limit of detection (LOD) of 10.778 µM. The calibration curves were linear in the concentration range 0.125 to 3.0 mM (R<sup>2</sup> = 0.99) for UA. Kinetic analysis revealed an electron transfer coefficient (α) of 0.390 and a diffusion coefficient (D) of D = 2.130 10<sup>−6</sup> cm<sup>2</sup> s<sup>−1</sup>. In addition, the ZnO/GCE demonstrated good repeatability and operational stability for uric acid detection. It also showed negligible interference from common biological species such as glucose and ascorbic acid. Overall, these results indicate that ZnO nanostructures on GCE represent a promising candidate material for electrochemical sensing of uric acid and potentially other analytes.</p>

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Electrochemical Determination of Uric Acid in Urine Using a ZnO-Modified Glassy Carbon Electrode

  • 期刊论文

  • 发表日期：

    2025年10月10日

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  • Nadia Ait Ahmed,
  • Katia Hebbache,
  • Katia Nasri,
  • Nabila Aliouane,
  • Atmane Djermoune,
  • Carine Chassigneux,
  • Marielle Eyraud

摘要

In this work, ZnO (zinc oxide) nanostructures were electrodeposited onto a glassy carbon electrode (GCE) using a simple, environmentally friendly, and low-cost electrochemical method. The electrocatalytic oxidation of uric acid (UA) was investigated at this ZnO/GCE. Electrochemical oxidation of uric acid in 0.1 M phosphate buffer solution PBS (0.1M K2HPO4 + 0.1 M KH2PO4) at pH = 7 was examined by cyclic voltammetry. The formation and structure of the ZnO/GCE were systematically characterized by SEM, EDX and XRD. These analyses showed that the synthetized ZnO nanostructures possessed good-crystallinity with well-defined nanorod morphology and favorable characteristics. The electrochemical response of the as-prepared ZnO-based sensor toward uric acid in PBS was further evaluated by linear sweep voltammetry (LSV) and chronoamperometry. The results confirmed that ZnO-modified GCE exhibited high electrocatalytic activity toward uric acid oxidation. Furthermore, the sensor demonstrated a high sensitivity of 28.4 µA µM−1 cm−2 and a low limit of detection (LOD) of 10.778 µM. The calibration curves were linear in the concentration range 0.125 to 3.0 mM (R2 = 0.99) for UA. Kinetic analysis revealed an electron transfer coefficient (α) of 0.390 and a diffusion coefficient (D) of D = 2.130 10−6 cm2 s−1. In addition, the ZnO/GCE demonstrated good repeatability and operational stability for uric acid detection. It also showed negligible interference from common biological species such as glucose and ascorbic acid. Overall, these results indicate that ZnO nanostructures on GCE represent a promising candidate material for electrochemical sensing of uric acid and potentially other analytes.