Employing lemon as a green fuel, zinc copper ferrites were synthesized in the current study utilizing the traditional solution combustion technique. The zinc copper ferrites nanoparticles have an average crystallite size of \(\:\sim\) 23 nm that are confirmed by X-Ray Diffraction (XRD) analysis. UV-diffuse reflectance spectra (DRS) analysis exhibits the bandgap of 2.75 eV and Scanning electron microscopy (SEM) & Energy Dispersive X-ray Analysis (EDAX) analysis reveals the agglomerated particles morphology and homogenous distribution of copper, zinc and iron within the crystal lattice. It is possible to discover that zinc copper ferrites nanoparticles exhibit superior activity when used as an effective material for electrochemical research by using carbon paste electrode and screen-printing electrode. The research findings are validated by their potential applications using cyclic voltammetry (CV) and amperometric (I-t) sensing techniques for the detection of biomolecules like ascorbic acid, creatinine, glucose, lactose, maltose and G-protein and heavy metals like mercury, cobalt as environmental components in 1 to 5 mM concentration. Further, simultaneous detection of two different biomolecules (maltose and G-protein) and heavy metal ions (mercury and cobalt) were carried out. The limit of detection (LOD) and limit of quantification (LOQ) have been determined for sensing of biomolecules and heavy metal ions. The results indicates that, zinc copper ferrites nanoparticles modified carbon paste electrode exhibit superior electrochemical sensing performance.
