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<p>Tuberose is commercially important for its fragrant flowers, with increasing demand for novel-coloured varieties in the ornamental industry. Meeting this demand, along with the need for disease-free planting material, requires an efficient mass multiplication system. The present study established a reproducible in vitro organogenesis and shoot regeneration protocol for the coloured tuberose cultivars ‘Pink Double’ and ‘Yellow Single’. Shoot tip and stem disc explants were cultured on Murashige and Skoog (MS) medium supplemented with plant growth regulators. Stem disc explants exhibited the highest regeneration efficiency and were therefore used in subsequent experiments. The maximum shoot regeneration frequency was obtained on MS medium containing 17.75 µM 6-Benzylaminopurine (BAP), 2.68 µM 1-Naphthaleneacetic acid (NAA), and 2.70 µM Thidiazuron (TDZ) for ‘Pink Double’ (94.03%), and 26.63 µM BAP, 2.68 µM NAA, and 2.70 µM TDZ for ‘Yellow Single’ (91.50%). For shoot multiplication, ‘Pink Double’ produced an average of 4.90 shoots per explant on MS medium with 17.75 µM BAP, 2.68 µM NAA, and 2.88 µM Gibberellic acid (GA<sub>3</sub>), while ‘Yellow Single’ produced 4.40 shoots on medium containing 26.63 µM BAP, 2.68 µM NAA, and 2.88 µM GA<sub>3</sub>. The highest rhizogenic response was obtained on half-strength MS medium supplemented with 25 µM Polyvinylpyrrolidone (PVP) and 4.92 µM Indole-3-butyric acid (IBA) in ‘Pink Double’, and 25 µM PVP with 7.38 µM IBA in ‘Yellow Single’. These treatments resulted in the earliest root initiation (20.50 and 25.40 days), the highest number of roots per explant (13.65 and 11.50) and the greatest root length (7.10 and 7.35&#xa0;cm) in ‘Pink Double’ and ‘Yellow Single’, respectively. Arka Fermented Cocopeat proved to be the most effective substrate for acclimatization of rooted plantlets, resulting in improved survival (100%) and growth. The optimized protocol developed in this study facilitates rapid multiplication and large-scale production of disease-free, high-quality planting material of coloured tuberose cultivars.</p> Graphical Abstract <p></p>

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Optimizing in vitro organogenesis and shoot regeneration of coloured tuberose (Agave amica) cultivars ‘Pink Double’ and ‘Yellow Single’

  • 期刊论文

  • 发表日期：

    2026年7月9日

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  • K. R. Nivya,
  • P. Nandeesha,
  • T. R. Usharani,
  • Sujatha A. Nair,
  • Rajiv Kumar,
  • Malleshaiah SharathKumar,
  • T. Usha Bharathi

摘要

Tuberose is commercially important for its fragrant flowers, with increasing demand for novel-coloured varieties in the ornamental industry. Meeting this demand, along with the need for disease-free planting material, requires an efficient mass multiplication system. The present study established a reproducible in vitro organogenesis and shoot regeneration protocol for the coloured tuberose cultivars ‘Pink Double’ and ‘Yellow Single’. Shoot tip and stem disc explants were cultured on Murashige and Skoog (MS) medium supplemented with plant growth regulators. Stem disc explants exhibited the highest regeneration efficiency and were therefore used in subsequent experiments. The maximum shoot regeneration frequency was obtained on MS medium containing 17.75 µM 6-Benzylaminopurine (BAP), 2.68 µM 1-Naphthaleneacetic acid (NAA), and 2.70 µM Thidiazuron (TDZ) for ‘Pink Double’ (94.03%), and 26.63 µM BAP, 2.68 µM NAA, and 2.70 µM TDZ for ‘Yellow Single’ (91.50%). For shoot multiplication, ‘Pink Double’ produced an average of 4.90 shoots per explant on MS medium with 17.75 µM BAP, 2.68 µM NAA, and 2.88 µM Gibberellic acid (GA3), while ‘Yellow Single’ produced 4.40 shoots on medium containing 26.63 µM BAP, 2.68 µM NAA, and 2.88 µM GA3. The highest rhizogenic response was obtained on half-strength MS medium supplemented with 25 µM Polyvinylpyrrolidone (PVP) and 4.92 µM Indole-3-butyric acid (IBA) in ‘Pink Double’, and 25 µM PVP with 7.38 µM IBA in ‘Yellow Single’. These treatments resulted in the earliest root initiation (20.50 and 25.40 days), the highest number of roots per explant (13.65 and 11.50) and the greatest root length (7.10 and 7.35 cm) in ‘Pink Double’ and ‘Yellow Single’, respectively. Arka Fermented Cocopeat proved to be the most effective substrate for acclimatization of rooted plantlets, resulting in improved survival (100%) and growth. The optimized protocol developed in this study facilitates rapid multiplication and large-scale production of disease-free, high-quality planting material of coloured tuberose cultivars.

Graphical Abstract