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<p><i>Hibiscus hamabo</i> Sieb. et Zucc., a typical halophyte species, holds great potential for use in saline-alkali land development and serves as an important material for studying the molecular mechanisms of transcriptional regulation of salt stress tolerance in woody plants. Plant stress responses, including salt tolerance, are significantly influenced by NAC proteins, which are transcription factors unique to plants. Building on previous studies, we used transcriptome sequencing in conjunction with gene silencing to identify important downstream genes regulated by HhNAC54. Furthermore, bimolecular fluorescence complementation (BiFC) and yeast hybrid assays were used to confirm the interaction between HhNAC54 and HhMYB73. Phenotypic identification of gene-silenced <i>H. hamabo</i> revealed that HhNAC54 had a positive regulatory function in salt tolerance. Physiological indicator assessment and transcriptomic data suggested that HhNAC54 may regulate downstream genes related to stress-responsive metabolic pathways, antioxidant defense and other processes. A total of 34 interacting proteins including MYB transcription factors, F-box/kelch-repeat protein, metallothionein-like protein, IQ-DOMAIN 14 protein, were screened from the cDNA library of salt treated <i>Hibiscus hamabo</i> Sieb. et Zucc. seedlings using yeast two-hybrid technology. In vivo verification via BiFC confirmed that the HhMYB73 protein, whose expression was induced under salt stress, interacts with HhNAC54. Collectively, these findings indicated that HhNAC54 may enhance salt tolerance by regulating metabolic and redox pathways while interacting with HhMYB73 to coordinate transcriptional responses under stress. This study established a theoretical basis for a more thorough comprehension of salt tolerance in <i>H. hamabo</i> and offered HhNAC54 and HhMYB73 as promising molecular targets for breeding or genetic engineering strategies aimed at improving salt tolerance in <i>Hibiscus</i> and potentially other woody crops.</p>

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HhNAC54, a transcription factor interacting with HhMYB73, enhances salt stress tolerance in Hibiscus hamabo Sieb. et Zucc.

  • 期刊论文

  • 发表日期：

    2026年6月24日

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  • Jinhong Li,
  • Qing Luo,
  • Luomin Cui,
  • Chaoguang Yu,
  • Jianfeng Hua,
  • Yunlong Yin,
  • Chunsun Gu,
  • Zhiquan Wang

摘要

Hibiscus hamabo Sieb. et Zucc., a typical halophyte species, holds great potential for use in saline-alkali land development and serves as an important material for studying the molecular mechanisms of transcriptional regulation of salt stress tolerance in woody plants. Plant stress responses, including salt tolerance, are significantly influenced by NAC proteins, which are transcription factors unique to plants. Building on previous studies, we used transcriptome sequencing in conjunction with gene silencing to identify important downstream genes regulated by HhNAC54. Furthermore, bimolecular fluorescence complementation (BiFC) and yeast hybrid assays were used to confirm the interaction between HhNAC54 and HhMYB73. Phenotypic identification of gene-silenced H. hamabo revealed that HhNAC54 had a positive regulatory function in salt tolerance. Physiological indicator assessment and transcriptomic data suggested that HhNAC54 may regulate downstream genes related to stress-responsive metabolic pathways, antioxidant defense and other processes. A total of 34 interacting proteins including MYB transcription factors, F-box/kelch-repeat protein, metallothionein-like protein, IQ-DOMAIN 14 protein, were screened from the cDNA library of salt treated Hibiscus hamabo Sieb. et Zucc. seedlings using yeast two-hybrid technology. In vivo verification via BiFC confirmed that the HhMYB73 protein, whose expression was induced under salt stress, interacts with HhNAC54. Collectively, these findings indicated that HhNAC54 may enhance salt tolerance by regulating metabolic and redox pathways while interacting with HhMYB73 to coordinate transcriptional responses under stress. This study established a theoretical basis for a more thorough comprehension of salt tolerance in H. hamabo and offered HhNAC54 and HhMYB73 as promising molecular targets for breeding or genetic engineering strategies aimed at improving salt tolerance in Hibiscus and potentially other woody crops.