Scholarlink

<p>Castor bean (<i>Ricinus communis</i> L.) recalcitrance limits biotechnological improvement. Thidiazuron (TDZ) based protocols cause hyperhydric bud abortion, while genotype dependency compromises reproducibility. We optimized the benzyladenine (BA)/indole-3-butyric acid (IBA) balance and investigated shoot apical meristem dependency using mature embryo axes of cultivar. Tongbi 5. The optimal combination of 1.5&#xa0;mg·L⁻¹ BA + 0.05&#xa0;mg·L⁻¹ IBA induced healthy adventitious shoots with efficiencies of 27.8 ± 1.9% via direct induction or 5.5 ± 1.9% via friable callus. SAM excision abolished bud formation, revealing SAM-dependent organogenic competence. BA alone produced green callus, which upon further culture yielded more abundant buds (9.67/explant) but severe hyperhydricity, whereas TDZ + IBA maximized bud number (15.3/explant, 65.6%) but compromised quality. Elevating IBA to 0.1&#xa0;mg·L⁻¹ or using 2.0&#xa0;mg·L⁻¹ BA + 0.8&#xa0;mg·L⁻¹ naphthaleneacetic acid (NAA) redirected differentiation toward rooting. Shoot elongation on 0.2&#xa0;mg·L⁻¹ BA achieved &gt; 90% elongation; rooting on 0.05&#xa0;mg·L⁻¹ IBA + 1% (w/v) activated charcoal yielded 81.1% efficiency and 88.9 ± 5.1% plantlet survival. This SAM-sensitive protocol, optimized for cv. Tongbi 5, provides a foundation for future transformation studies in castor bean.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Shoot apical meristem-dependent adventitious bud induction from embryo axes of castor bean (Ricinus communis L.) via optimized cytokinin-auxin ratio

  • 期刊论文

  • 发表日期：

    2026年5月6日

查看全文
  • Liang Bai,
  • Liying Shan,
  • Jian Song,
  • Mengchen Xu,
  • Yue Wang,
  • Ruiyan Guo,
  • Yanli Jin,
  • Yangguang Duan,
  • Yuling Zhang,
  • Zhimin Jiang,
  • Jilong Zhong,
  • Yan Cheng

摘要

Castor bean (Ricinus communis L.) recalcitrance limits biotechnological improvement. Thidiazuron (TDZ) based protocols cause hyperhydric bud abortion, while genotype dependency compromises reproducibility. We optimized the benzyladenine (BA)/indole-3-butyric acid (IBA) balance and investigated shoot apical meristem dependency using mature embryo axes of cultivar. Tongbi 5. The optimal combination of 1.5 mg·L⁻¹ BA + 0.05 mg·L⁻¹ IBA induced healthy adventitious shoots with efficiencies of 27.8 ± 1.9% via direct induction or 5.5 ± 1.9% via friable callus. SAM excision abolished bud formation, revealing SAM-dependent organogenic competence. BA alone produced green callus, which upon further culture yielded more abundant buds (9.67/explant) but severe hyperhydricity, whereas TDZ + IBA maximized bud number (15.3/explant, 65.6%) but compromised quality. Elevating IBA to 0.1 mg·L⁻¹ or using 2.0 mg·L⁻¹ BA + 0.8 mg·L⁻¹ naphthaleneacetic acid (NAA) redirected differentiation toward rooting. Shoot elongation on 0.2 mg·L⁻¹ BA achieved > 90% elongation; rooting on 0.05 mg·L⁻¹ IBA + 1% (w/v) activated charcoal yielded 81.1% efficiency and 88.9 ± 5.1% plantlet survival. This SAM-sensitive protocol, optimized for cv. Tongbi 5, provides a foundation for future transformation studies in castor bean.