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<p><i>Waltheria indica</i> L. is a traditional officinal plant with many medicinal and insecticidal properties. This study established an in vitro propagation system for <i>W. indica</i> and evaluated the repellent activity of the compounds from in vitro-derived plants against the nematode <i>Meloidogyne incognita</i>. We optimized the medium for axillary bud proliferation and rooting of sterile shoots. Adventitious buds were induced from hypocotyls on Murashige and Skoog (MS) + 0.1&#xa0;mg/L 6-benzyladenine (BA). MS + 1.0&#xa0;mg/L 6-BA + 0.2&#xa0;mg/L 1-napthaleneacetic acid (NAA) + 1.0&#xa0;mg/L 2,4-dichlorophenoxyacetic acid was the best media for adventitious root induction from callus. An adventitious root suspension culture system was successfully induced and established in MS medium supplemented with 1.0&#xa0;mg/L NAA, using a 1% inoculum size and a 30-day subculture interval. We isolated and identified antidesmone and trans-tiliroside from <i>W. indica</i> plants. They demonstrated repellent activity against J2 of <i>M. incognita</i> with chemotaxis index of 0.41 and 0.22, respectively, at a concentration of 10&#xa0;mg/L. The relative content of antidesmone in adventitious roots cultured in liquid suspension was significantly higher than that in tissue-cultured plantlets. Exogenous supplementation of 0.05&#xa0;mg/L 6-BA significantly improved the accumulation of trans-tiliroside synthesis in stems and leaves by reversing the inhibitory effect of tissue culture conditions. This study provides a theoretical basis and technical support for the large-scale propagation, germplasm improvement, and regulation of active secondary metabolite production in <i>W. indica.</i></p>

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Establishment of an in vitro propagation system to acquisition of nematode-repellent secondary metabolites of Waltheria indica L.

  • 期刊论文

  • 发表日期：

    2026年4月13日

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  • Meihua Tang,
  • Xinyi Lu,
  • Yongdie Gan,
  • Sher Ahmed,
  • Zhan Hu,
  • Dong Li,
  • Shuai Wang,
  • Ranfeng Sun

摘要

Waltheria indica L. is a traditional officinal plant with many medicinal and insecticidal properties. This study established an in vitro propagation system for W. indica and evaluated the repellent activity of the compounds from in vitro-derived plants against the nematode Meloidogyne incognita. We optimized the medium for axillary bud proliferation and rooting of sterile shoots. Adventitious buds were induced from hypocotyls on Murashige and Skoog (MS) + 0.1 mg/L 6-benzyladenine (BA). MS + 1.0 mg/L 6-BA + 0.2 mg/L 1-napthaleneacetic acid (NAA) + 1.0 mg/L 2,4-dichlorophenoxyacetic acid was the best media for adventitious root induction from callus. An adventitious root suspension culture system was successfully induced and established in MS medium supplemented with 1.0 mg/L NAA, using a 1% inoculum size and a 30-day subculture interval. We isolated and identified antidesmone and trans-tiliroside from W. indica plants. They demonstrated repellent activity against J2 of M. incognita with chemotaxis index of 0.41 and 0.22, respectively, at a concentration of 10 mg/L. The relative content of antidesmone in adventitious roots cultured in liquid suspension was significantly higher than that in tissue-cultured plantlets. Exogenous supplementation of 0.05 mg/L 6-BA significantly improved the accumulation of trans-tiliroside synthesis in stems and leaves by reversing the inhibitory effect of tissue culture conditions. This study provides a theoretical basis and technical support for the large-scale propagation, germplasm improvement, and regulation of active secondary metabolite production in W. indica.