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<p>African marigold (<i>Tagetes erecta</i> L.) (family: Asteraceae) is an ornamental plant renowned for both its aesthetic value and therapeutic properties, and it holds a significant place in traditional Ayurvedic medicine. Its industrial importance is largely attributed to the presence of lutein (in flower petals), a natural dietary pigment with diverse commercial applications. Moreover, marigold exhibits insecticidal and nematocidal activities. The successful establishment of aseptic cultures is, therefore, a major step toward exploring its biotechnological and pharmaceutical potential, considering the limitations in its propagation via seeds or cuttings. The present study was aimed at developing an efficient protocol for mono-phasic (simultaneous shoot-root formation) mass propagation of true-to-type African marigold along with lutein production. Three cytokinins, viz. 6-benzyladenine (BA), kinetin (Kn), and <i>meta</i>-Topolin (<i>m</i>T) were utilized with Murashige and Skoog medium at varying concentrations ranging from 0.25 to 1.25&#xa0;mg/L. Maximum shoot proliferation (4.3 shoots per explant) was obtained with 0.25&#xa0;mg/L <i>m</i>T. Simultaneous root induction was also observed in the same treatment, indicating an accelerated in vitro regeneration system for marigold. Among the cytokinins tested, 0.25&#xa0;mg/L <i>m</i>T induced the maximum number (6.3) and 0.25 mg/L BA induced maximum length of roots (7.3&#xa0;cm). The in vitro–regenerated plantlets were successfully acclimatized, fully grown, and flowered in a soil-sand mixture (1:1, v/v), achieving a survival rate of 85%. The genetic fidelity of in vitro–regenerated plantlets was assessed through a combination of micromorphological analysis, flow cytometry, and molecular markers using inter simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphism primers. Lutein content in the petals was quantified using high-performance liquid chromatography, which revealed that control (mother) plant contained 3.83&#xa0;mg/g dry weight (DW) of lutein, whereas the vitro–regenerated plants exhibited a little higher accumulation of 3.91&#xa0;mg/g DW.</p>

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meta-Topolin-induced in vitro monophasic propagation, floral development, and fidelity analysis of African marigold

  • 期刊论文

  • 发表日期：

    2026年2月25日

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  • Anwesa Bhusan,
  • Jayoti Majumder Sarkar,
  • Saikat Gantait,
  • Shraddha Singh,
  • Sudhir Singh,
  • Somnath Bhattacharyya

摘要

African marigold (Tagetes erecta L.) (family: Asteraceae) is an ornamental plant renowned for both its aesthetic value and therapeutic properties, and it holds a significant place in traditional Ayurvedic medicine. Its industrial importance is largely attributed to the presence of lutein (in flower petals), a natural dietary pigment with diverse commercial applications. Moreover, marigold exhibits insecticidal and nematocidal activities. The successful establishment of aseptic cultures is, therefore, a major step toward exploring its biotechnological and pharmaceutical potential, considering the limitations in its propagation via seeds or cuttings. The present study was aimed at developing an efficient protocol for mono-phasic (simultaneous shoot-root formation) mass propagation of true-to-type African marigold along with lutein production. Three cytokinins, viz. 6-benzyladenine (BA), kinetin (Kn), and meta-Topolin (mT) were utilized with Murashige and Skoog medium at varying concentrations ranging from 0.25 to 1.25 mg/L. Maximum shoot proliferation (4.3 shoots per explant) was obtained with 0.25 mg/L mT. Simultaneous root induction was also observed in the same treatment, indicating an accelerated in vitro regeneration system for marigold. Among the cytokinins tested, 0.25 mg/L mT induced the maximum number (6.3) and 0.25 mg/L BA induced maximum length of roots (7.3 cm). The in vitro–regenerated plantlets were successfully acclimatized, fully grown, and flowered in a soil-sand mixture (1:1, v/v), achieving a survival rate of 85%. The genetic fidelity of in vitro–regenerated plantlets was assessed through a combination of micromorphological analysis, flow cytometry, and molecular markers using inter simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphism primers. Lutein content in the petals was quantified using high-performance liquid chromatography, which revealed that control (mother) plant contained 3.83 mg/g dry weight (DW) of lutein, whereas the vitro–regenerated plants exhibited a little higher accumulation of 3.91 mg/g DW.