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<p>The floriculture industry continually seeks new varieties of flowering plants that can adapt to changing environments, thrive in diverse locations, and exhibit attractive flowers or inflorescences, while maintaining their unique characteristics. This study involves a comprehensive analysis of <i>Eulophia nuda</i> Lindl. (1833:180) and <i>Eulophia andamanensis</i> Rchb.f. (1872: 276), focusing on ornamental potential, asymbiotic seed germination, direct protocorm proliferation and mass multiplication. Delving into these areas will uncover the untapped potential of genus <i>Eulophia</i>. Ornamental potential assessment of both species was evaluated based on twenty-four traits, such as foliage on the plant, length of inflorescence, total flowers on inflorescence, etc. <i>E. nuda</i> features hypogeal bulbs and linear leaves, giving it a palm-like appearance. While <i>E. andamanensis</i> has epigeal pseudobulbs and fleshy linear-lanceolate leaves, and have almost three times longer to bloom than <i>E. nuda</i>, highlighting its ornamental potential as a pot plant. In addition, in vitro asymbiotic seed germination studies were conducted using four different semi-solid media at 3 different concentrations devoid of growth regulators, and the growth performance was assessed using two plant growth regulators, IBA and BAP, at concentrations of 0.5 mg/l to 2.0 mg/l. Full-strength Knudson C medium showed the highest germination (93.76±0.65%) in <i>E. nuda</i> and (94.29±11%) in <i>E. andamnensis,</i> highlighting slightly better in vitro adaptability in <i>E. nuda</i>. In comparing seed germination media, both species require Kn. C medium with varying concentrations of IBA and BAP (0.5 to 2.0 mg/L). Lower concentrations promote protocorm development, while higher concentrations (1.0 to 2.0 mg/L) support protocorm development into microbulbs with roots and shoots. However, concentrations of 2.0 mg/L may negatively impact protocorms. However, <i>E. andamnensis</i> demonstrated superior floral traits under ex vitro conditions. MS medium supplemented with different concentrations of IAA (1.0–2.0 mg/l) was found to be optimum for protocorms proliferation. For the proliferation of the protocorms in well-rooted and good shoot bearing plant, adding MS medium with 1·5 mg/l IAA produced effective results in both species. The rooted plantlets acclimatized to the greenhouse show an 80% survival rate.</p>

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In vitro asymbiotic seed germination, mass multiplication and evaluation of ornamental traits in wild orchids: a comparative study of Eulophia nuda Lindl. and Eulophia andamanensis Rchb.f.

  • 期刊论文

  • 发表日期：

    2026年3月5日

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  • Mayur S. Jadhav,
  • Sobiyanaz A. Momin,
  • Rajaram V. Gurav

摘要

The floriculture industry continually seeks new varieties of flowering plants that can adapt to changing environments, thrive in diverse locations, and exhibit attractive flowers or inflorescences, while maintaining their unique characteristics. This study involves a comprehensive analysis of Eulophia nuda Lindl. (1833:180) and Eulophia andamanensis Rchb.f. (1872: 276), focusing on ornamental potential, asymbiotic seed germination, direct protocorm proliferation and mass multiplication. Delving into these areas will uncover the untapped potential of genus Eulophia. Ornamental potential assessment of both species was evaluated based on twenty-four traits, such as foliage on the plant, length of inflorescence, total flowers on inflorescence, etc. E. nuda features hypogeal bulbs and linear leaves, giving it a palm-like appearance. While E. andamanensis has epigeal pseudobulbs and fleshy linear-lanceolate leaves, and have almost three times longer to bloom than E. nuda, highlighting its ornamental potential as a pot plant. In addition, in vitro asymbiotic seed germination studies were conducted using four different semi-solid media at 3 different concentrations devoid of growth regulators, and the growth performance was assessed using two plant growth regulators, IBA and BAP, at concentrations of 0.5 mg/l to 2.0 mg/l. Full-strength Knudson C medium showed the highest germination (93.76±0.65%) in E. nuda and (94.29±11%) in E. andamnensis, highlighting slightly better in vitro adaptability in E. nuda. In comparing seed germination media, both species require Kn. C medium with varying concentrations of IBA and BAP (0.5 to 2.0 mg/L). Lower concentrations promote protocorm development, while higher concentrations (1.0 to 2.0 mg/L) support protocorm development into microbulbs with roots and shoots. However, concentrations of 2.0 mg/L may negatively impact protocorms. However, E. andamnensis demonstrated superior floral traits under ex vitro conditions. MS medium supplemented with different concentrations of IAA (1.0–2.0 mg/l) was found to be optimum for protocorms proliferation. For the proliferation of the protocorms in well-rooted and good shoot bearing plant, adding MS medium with 1·5 mg/l IAA produced effective results in both species. The rooted plantlets acclimatized to the greenhouse show an 80% survival rate.