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<p>Extraction of paclitaxel (PTX) from <i>Corylus avellana</i> cell cultures have been widely demonstrated, though with limited yield. This study explores the use of aluminum oxide (Al<sub>2</sub>O<sub>2</sub>) and tungsten oxide (WO<sub>3</sub>) nanoparticles as novel elicitors to enhance paclitaxel production by leaf-derived <i>Corylus avellana</i> calli. The calli were obtained from leaf explants in Murashige and Skoog medium supplemented with varying concentrations of auxin and cytokinin, and were sub-cultured every 21 days. Two kinds of auxins (NAA and IAA in equal amounts) were used in each treatment). After getting a line, the calli were treated with different concentrations (0, 2.5, 5, 10&#xa0;mg. L<sup>− 1</sup>) of Al<sub>2</sub>O<sub>3</sub> and WO<sub>3</sub> nanoparticles (NPs) at their logarithmic growth phase. Paclitaxel was extracted and quantified via HPLC, while gene expression levels of key biosynthetic enzymes i.e., Taxadiene synthase (TS), Deacetylbaccatin III-10-O-acetyltransferase (DBAT), and phenylalanine aminomutase (PAM) were analyzed using RT-qPCR. The results demonstrated that nanoparticles significantly increased biomass and paclitaxel yield, with optimal enhancements specifically at 10&#xa0;mg. L<sup>− 1</sup> of Al<sub>2</sub>O<sub>3</sub> and 5&#xa0;mg. L<sup>−1</sup>of WO<sub>3</sub>. It was accompanied by remarkable upregulation of taxane biosynthetic genes. These findings highlight the potential of Al<sub>2</sub>O<sub>3</sub> and WO<sub>3</sub> nanoparticles as effective elicitors for scalable and sustainable paclitaxel production in hazel cultures, and offer promising biotechnological implications for pharmaceutical applications.</p>

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Enhancement of taxans production via Al2O3NPs and WO3 NPs treatment of leaf-derived callus of Corylus avellana

  • 期刊论文

  • 发表日期：

    2026年2月24日

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  • Baan Munim Twaij,
  • Faezeh Ghanati,
  • Hashim K. Mohammed Al-Oubaidi

摘要

Extraction of paclitaxel (PTX) from Corylus avellana cell cultures have been widely demonstrated, though with limited yield. This study explores the use of aluminum oxide (Al2O2) and tungsten oxide (WO3) nanoparticles as novel elicitors to enhance paclitaxel production by leaf-derived Corylus avellana calli. The calli were obtained from leaf explants in Murashige and Skoog medium supplemented with varying concentrations of auxin and cytokinin, and were sub-cultured every 21 days. Two kinds of auxins (NAA and IAA in equal amounts) were used in each treatment). After getting a line, the calli were treated with different concentrations (0, 2.5, 5, 10 mg. L− 1) of Al2O3 and WO3 nanoparticles (NPs) at their logarithmic growth phase. Paclitaxel was extracted and quantified via HPLC, while gene expression levels of key biosynthetic enzymes i.e., Taxadiene synthase (TS), Deacetylbaccatin III-10-O-acetyltransferase (DBAT), and phenylalanine aminomutase (PAM) were analyzed using RT-qPCR. The results demonstrated that nanoparticles significantly increased biomass and paclitaxel yield, with optimal enhancements specifically at 10 mg. L− 1 of Al2O3 and 5 mg. L−1of WO3. It was accompanied by remarkable upregulation of taxane biosynthetic genes. These findings highlight the potential of Al2O3 and WO3 nanoparticles as effective elicitors for scalable and sustainable paclitaxel production in hazel cultures, and offer promising biotechnological implications for pharmaceutical applications.