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<p><i>Coelogyne mossiae</i> is an endangered and endemic orchid of the Western Ghats, India, yet its conservation and pharmaceutical potential remain largely unexplored. The present investigation aimed to develop an efficient in vitro regeneration protocol through Protocorm-Like bodies (PLBs) and to evaluate the bioactive properties of the regenerated plants. Bisected pseudobulb explants yielded a maximum PLB frequency of 72.80%, producing an average of 11.70 PLBs, of which 3.85 successfully developed into plantlets after 85 days of culture on MS medium supplemented with 1.0&#xa0;mg/L 2,4-D and TDZ. Genetic fidelity assessment using SCoT markers confirmed the clonal stability of the regenerated plantlets. Methanolic extracts prepared from one-year-old greenhouse-grown plants revealed the presence of bioactive compounds, as evidenced by FT-IR and GC-MS analyses. Furthermore, the extracts demonstrated notable antioxidant activity (DPPH and Hydroxyl radical scavenging assays) and antibacterial efficacy, reflected by substantial zones of inhibition against selected bacterial strains. Overall, this study provides a reliable mass propagation protocol for <i>C. mossiae</i> and highlights its promising pharmaceutical potential.</p>

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Optimizing a standard protocol for protocorm-like bodies induction and mass propagation of Coelogyne mossiae plantlets for efficient evaluation of pharmaceutical potential

  • 期刊论文

  • 发表日期：

    2026年2月3日

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  • Shivakrishnan Kaladharan,
  • Palaniyappan Subramanian,
  • Rafia Akhtar,
  • Senthil Kumar Thiruppathi

摘要

Coelogyne mossiae is an endangered and endemic orchid of the Western Ghats, India, yet its conservation and pharmaceutical potential remain largely unexplored. The present investigation aimed to develop an efficient in vitro regeneration protocol through Protocorm-Like bodies (PLBs) and to evaluate the bioactive properties of the regenerated plants. Bisected pseudobulb explants yielded a maximum PLB frequency of 72.80%, producing an average of 11.70 PLBs, of which 3.85 successfully developed into plantlets after 85 days of culture on MS medium supplemented with 1.0 mg/L 2,4-D and TDZ. Genetic fidelity assessment using SCoT markers confirmed the clonal stability of the regenerated plantlets. Methanolic extracts prepared from one-year-old greenhouse-grown plants revealed the presence of bioactive compounds, as evidenced by FT-IR and GC-MS analyses. Furthermore, the extracts demonstrated notable antioxidant activity (DPPH and Hydroxyl radical scavenging assays) and antibacterial efficacy, reflected by substantial zones of inhibition against selected bacterial strains. Overall, this study provides a reliable mass propagation protocol for C. mossiae and highlights its promising pharmaceutical potential.