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<p><i>Coccinia grandis</i> (L.) Voigt is a medicinal plant recognized for its wide range of pharmacological properties. The present study focused on optimizing cell suspension cultures (CSCs) of <i>C. grandis</i> to enhance the production of 2-methoxy-4-vinylphenol (2M4VP), an anticancer compound. The individual effects of precursor feeding with L-phenylalanine (PHE) and L-tyrosine (TYS), as well as biotic elicitation using yeast extract (YE) and chitin (CT), were systematically evaluated. Among all treatments, 150 µM PHE produced the most significant enhancement, resulting in a 1.98-fold increase in fresh weight (11.98 ± 0.025&#xa0;g FW), a 4.39-fold increase in dry weight (1.99 ± 0.005&#xa0;g DW), and a 5.53-fold increase in 2M4VP content (5.31 ± 0.04 mg g<sup>− 1</sup> DW) compared to the corresponding controls. Antioxidant potential, evaluated through 2,2-Diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical scavenging assay (HRSA), and 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS<sup>+</sup>). Under 150 µM PHE treatment, the antioxidant potential was significantly enhanced, showing 1.32-, 1.90-, and 1.03-fold increases, respectively, against the control. Overall, the findings indicated that precursor feeding, particularly with PHE, was more effective than biotic elicitation in enhancing biomass accumulation and 2M4VP production in <i>C. grandis</i> CSCs. This approach offers a scalable and reliable platform for producing pharmaceutical 2M4VP, an important anticancer compound.</p>

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Unlocking the biosynthetic potential of Coccinia grandis (L.) Voigt through targeted elicitation of 2M4VP via precursors and biotic elicitors

  • 期刊论文

  • 发表日期：

    2026年1月26日

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  • Ananthakumar Archana,
  • Shanthi Pandurengan Parthasarathy,
  • Subramaniyam Alagumanian,
  • Markandan Manickavasagam

摘要

Coccinia grandis (L.) Voigt is a medicinal plant recognized for its wide range of pharmacological properties. The present study focused on optimizing cell suspension cultures (CSCs) of C. grandis to enhance the production of 2-methoxy-4-vinylphenol (2M4VP), an anticancer compound. The individual effects of precursor feeding with L-phenylalanine (PHE) and L-tyrosine (TYS), as well as biotic elicitation using yeast extract (YE) and chitin (CT), were systematically evaluated. Among all treatments, 150 µM PHE produced the most significant enhancement, resulting in a 1.98-fold increase in fresh weight (11.98 ± 0.025 g FW), a 4.39-fold increase in dry weight (1.99 ± 0.005 g DW), and a 5.53-fold increase in 2M4VP content (5.31 ± 0.04 mg g− 1 DW) compared to the corresponding controls. Antioxidant potential, evaluated through 2,2-Diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical scavenging assay (HRSA), and 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+). Under 150 µM PHE treatment, the antioxidant potential was significantly enhanced, showing 1.32-, 1.90-, and 1.03-fold increases, respectively, against the control. Overall, the findings indicated that precursor feeding, particularly with PHE, was more effective than biotic elicitation in enhancing biomass accumulation and 2M4VP production in C. grandis CSCs. This approach offers a scalable and reliable platform for producing pharmaceutical 2M4VP, an important anticancer compound.