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<p>The AP2/ERF transcription factor family genes play crucial roles in plant growth, development, and response to abiotic stress. In this study, a highly expressed ERF family gene, <i>PsnERF1B</i>, under salt stress was screened, which is localized in the nucleus and lacks transcriptional activation activity. Yeast one-hybrid assays showed that the PsnERF1B protein could specifically bind to GCC-box elements. Under salt stress, overexpression of PsnERF1B significantly enhanced the activities of SOD and POD in transgenic poplar plants. In addition, transgenic lines exhibited higher chlorophyll and proline contents, accompanied by a marked reduction in malondialdehyde (MDA) levels, indicating decreased lipid peroxidation and improved cellular integrity. Consistent with these physiological changes, the expression levels of several POD- and SOD-related genes were significantly upregulated in the transgenic lines under salt stress, suggesting that PsnERF1B may promote salt tolerance by activating antioxidant defense pathways at both the transcriptional and physiological levels. This study offers preliminary insights into the molecular mechanisms of <i>PsnERF1B</i> gene in salt stress tolerance, which may contribute to future research in molecular biology and genetic transformation.</p>

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Functional characterization of PsnErf1b in regulating salt stress tolerance in poplar

  • 期刊论文

  • 发表日期：

    2025年12月29日

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  • Zihan Cheng,
  • Xinyu He,
  • Xinyue Tu,
  • Haiyan Fang,
  • Lin Liu,
  • Tingbo Jiang,
  • Xuemei Zhang

摘要

The AP2/ERF transcription factor family genes play crucial roles in plant growth, development, and response to abiotic stress. In this study, a highly expressed ERF family gene, PsnERF1B, under salt stress was screened, which is localized in the nucleus and lacks transcriptional activation activity. Yeast one-hybrid assays showed that the PsnERF1B protein could specifically bind to GCC-box elements. Under salt stress, overexpression of PsnERF1B significantly enhanced the activities of SOD and POD in transgenic poplar plants. In addition, transgenic lines exhibited higher chlorophyll and proline contents, accompanied by a marked reduction in malondialdehyde (MDA) levels, indicating decreased lipid peroxidation and improved cellular integrity. Consistent with these physiological changes, the expression levels of several POD- and SOD-related genes were significantly upregulated in the transgenic lines under salt stress, suggesting that PsnERF1B may promote salt tolerance by activating antioxidant defense pathways at both the transcriptional and physiological levels. This study offers preliminary insights into the molecular mechanisms of PsnERF1B gene in salt stress tolerance, which may contribute to future research in molecular biology and genetic transformation.