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<p>Chronic inflammation, characterized by sustained immune activation, is increasingly associated with various non-communicable diseases. In the current study, a cell suspension culture of <i>Andrographis paniculata</i> has been developed to evaluate its anti-inflammatory potential by assessing its effect on the expression of key pro-inflammatory cytokines viz. Tumor Necrosis Factor-alpha (TNF-α) and Interleukin-6 (IL-6). The cell suspension culture was derived from friable callus grown in Murashige and Skoog (MS) medium enriched with 3.0&#xa0;mg/L 2,4-D and 0.5&#xa0;mg/L BAP, and growth kinetics were monitored. The highest growth index (973 ± 126) was observed on the 45th day, which also corresponded with peak production of key bioactive compounds viz. andrographolide, neo-andrographolide, and 14-deoxy-11,12-didehydroandrographolide (14-DDA). Strong binding affinities to TNF-α and IL-6 were shown by molecular docking studies, with neo-andrographolide and andrographolide displaying the highest binding energies (-7.9&#xa0;kcal/mol with TNF-α and − 6.8&#xa0;kcal/mol with IL-6). Notably, neo-andrographolide formed five hydrogen bonds within the TNF-α binding pocket, indicating a strong interaction. In vitro anti-inflammatory activity analysis showed that elevated levels of TNF-α and IL-6 were observed in LPS-stimulated cells compared to non-stimulated controls. Treatment with cell suspension extract (1 and 10&#xa0;µg/mL) significantly suppressed pro-inflammatory cytokine production at the higher concentration (10&#xa0;µg/mL), demonstrating a dose-dependent anti-inflammatory effect. Overall, this study highlights the dual benefit of <i>A. paniculata</i> cell suspension cultures for both sustainable bioactive compound production and potential therapeutic application in the management of chronic inflammatory conditions.</p>

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Biotechnological approach to produce anti-inflammatory secondary metabolites in Andrographis paniculata via cell suspension culture

  • 期刊论文

  • 发表日期：

    2025年12月23日

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  • Pooja Singh,
  • Namita Gupta,
  • Aqib Sarfraz,
  • Zafar Iqbal,
  • Surbhi Bisht,
  • Dnyaneshwar Umrao Bawankule,
  • Sabika Akram,
  • Feroz Khan,
  • Laiq ur Rahman

摘要

Chronic inflammation, characterized by sustained immune activation, is increasingly associated with various non-communicable diseases. In the current study, a cell suspension culture of Andrographis paniculata has been developed to evaluate its anti-inflammatory potential by assessing its effect on the expression of key pro-inflammatory cytokines viz. Tumor Necrosis Factor-alpha (TNF-α) and Interleukin-6 (IL-6). The cell suspension culture was derived from friable callus grown in Murashige and Skoog (MS) medium enriched with 3.0 mg/L 2,4-D and 0.5 mg/L BAP, and growth kinetics were monitored. The highest growth index (973 ± 126) was observed on the 45th day, which also corresponded with peak production of key bioactive compounds viz. andrographolide, neo-andrographolide, and 14-deoxy-11,12-didehydroandrographolide (14-DDA). Strong binding affinities to TNF-α and IL-6 were shown by molecular docking studies, with neo-andrographolide and andrographolide displaying the highest binding energies (-7.9 kcal/mol with TNF-α and − 6.8 kcal/mol with IL-6). Notably, neo-andrographolide formed five hydrogen bonds within the TNF-α binding pocket, indicating a strong interaction. In vitro anti-inflammatory activity analysis showed that elevated levels of TNF-α and IL-6 were observed in LPS-stimulated cells compared to non-stimulated controls. Treatment with cell suspension extract (1 and 10 µg/mL) significantly suppressed pro-inflammatory cytokine production at the higher concentration (10 µg/mL), demonstrating a dose-dependent anti-inflammatory effect. Overall, this study highlights the dual benefit of A. paniculata cell suspension cultures for both sustainable bioactive compound production and potential therapeutic application in the management of chronic inflammatory conditions.