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<p><i>Acer truncatum</i> is an economically important multipurpose tree in China due to its high values in ornamental, ecology, oil-production and medicine. The lack of reliable and stable in vitro regeneration system seriously restricts its breeding and propagation. This study first presented a protocol for in vitro regeneration of <i>A. truncatum</i> via de novo shoot organogenesis from stem explants. The results showed that sterilization, basal medium, plant growth regulators and light condition significantly influenced shoot regeneration of <i>A. truncatum</i>. The best sterilization protocol for stem explants was 75% ethanol (30&#xa0;s) + 0.1% HgCl<sub>2</sub> (10&#xa0;min), with 4.74% contamination rate. The highest callus induction rate (97.17%) was achieved when stem segments were cultured on WPM medium with 0.2&#xa0;mg/L 2,4-D and 0.5&#xa0;mg/L 6-BA in the dark for 15 days. The highest shoot differentiation rate (10.51%) was obtained when callus was cultured on NN69 medium containing 0.5&#xa0;mg/L CPPU. The rooting rate of adventitious shoots was achieved 85% by using NN69 medium with 0.25&#xa0;mg/L IBA, and the rooted shoots were transplanted into pots containing a mixture of peat, perlite and vermiculite (2:1:1, v: v:v), the survival rate was more than 80%. Histological analysis demonstrated the origin and development process of indirect shoot organogenesis, including callus formation, meristematic nodules formation and shoots differentiation. This study will be beneficial to the germplasm conservation, mass propagation and genetic improvement of <i>A. truncatum</i>.</p>

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In vitro regeneration of Acer truncatum via shoot organogenesis from stem explants

  • 期刊论文

  • 发表日期：

    2025年12月18日

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  • Yiming Du,
  • Wenqi Zhao,
  • Meng Xin,
  • Shushun Li,
  • Lu Zhu,
  • Qiuyue Ma,
  • Kunyuan Yan,
  • Guojun Zhang,
  • Qianzhong Li

摘要

Acer truncatum is an economically important multipurpose tree in China due to its high values in ornamental, ecology, oil-production and medicine. The lack of reliable and stable in vitro regeneration system seriously restricts its breeding and propagation. This study first presented a protocol for in vitro regeneration of A. truncatum via de novo shoot organogenesis from stem explants. The results showed that sterilization, basal medium, plant growth regulators and light condition significantly influenced shoot regeneration of A. truncatum. The best sterilization protocol for stem explants was 75% ethanol (30 s) + 0.1% HgCl2 (10 min), with 4.74% contamination rate. The highest callus induction rate (97.17%) was achieved when stem segments were cultured on WPM medium with 0.2 mg/L 2,4-D and 0.5 mg/L 6-BA in the dark for 15 days. The highest shoot differentiation rate (10.51%) was obtained when callus was cultured on NN69 medium containing 0.5 mg/L CPPU. The rooting rate of adventitious shoots was achieved 85% by using NN69 medium with 0.25 mg/L IBA, and the rooted shoots were transplanted into pots containing a mixture of peat, perlite and vermiculite (2:1:1, v: v:v), the survival rate was more than 80%. Histological analysis demonstrated the origin and development process of indirect shoot organogenesis, including callus formation, meristematic nodules formation and shoots differentiation. This study will be beneficial to the germplasm conservation, mass propagation and genetic improvement of A. truncatum.