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<p>Stilbenes are bioactive molecules widely studied for their health benefits. A previously established <i>Vitis vinifera</i> hairy root line was used for its ability to produce several stilbenes, including <i>trans</i>-resveratrol, <i>trans</i>-piceatannol, <i>cis</i>-vitisin B, <i>trans</i>-piceid, pallidol, and <i>trans</i>-ε-viniferin. The influence of methyl-β-cyclodextrin (βCD) and Linecaps<sup>®</sup> maltodextrin was assessed during the growth phase, prior to elicitation (induced after a complete medium renewal). Stilbenes were extracted 7 days after elicitation from both the culture medium and the roots as crude extracts. Their antioxidant activities were evaluated using four in vitro assays (ABTS, DPPH, FRAP and CUPRAC) and their antimicrobial activities were tested on three skin-associated bacteria (<i>Acinetobacter calcoaceticus</i>, <i>Corynebacterium xerosis</i>, <i>Staphylococcus epidermis</i>). Cytotoxicity was assessed using an immortalized human keratinocyte line (HaCaT). Correlations between stilbenes content and bioactivity were examined. Roots pre-treated with βCD produced significantly more stilbenes (27,5&#xa0;mg/100&#xa0;mg crude extract) compared to positive control (10,3&#xa0;mg/100&#xa0;mg crude extract) and increased dimer formation (8&#xa0;mg Viniferin/100&#xa0;mg crude extract against 1,5&#xa0;mg Viniferin/100&#xa0;mg crude extract in positive control), suggesting a potentializing effect. In contrast, Linecaps-treated roots produced similar stilbenes levels than the positive control but showed a significant increase in roots biomass (1,5&#xa0;g of fresh biomass against 0,6&#xa0;g in positive control condition). Crude extracts from the culture medium cultured with βCD, rich in secreted stilbenes, exhibited the strongest antioxidant (ABTS: 0,5&#xa0;g Trolox / g extract; CUPRAC: 0,3&#xa0;g Trolox / g extract) and antimicrobial effects (IC50 = 0,310 mg.mL<sup>− 1</sup> for <i>Staphylococcus epidermis</i>), with similar cytotoxicity to less enriched extracts (IC50 = 0,864 mg.mL<sup>− 1</sup> compared to pure resveratrol which have an IC50 = 0,099 mg.mL<sup>− 1</sup>). This hairy root system proves to be a promising platform for producing bioactive plant compounds with potential cosmetic applications.</p>

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Antioxidant and antimicrobial capacities of complex organic extracts containing stilbenes derivatives obtained from Vitis vinifera Pinot Noir hairy roots cultures for potential application in cosmetics

  • 期刊论文

  • 发表日期：

    2025年12月15日

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  • Alban Coquel,
  • Elodie Choque,
  • Romain Roulard,
  • Sandy Theysgeur,
  • Isabelle Gaillard,
  • Rozenn Ravallec,
  • Nathalie Jullian

摘要

Stilbenes are bioactive molecules widely studied for their health benefits. A previously established Vitis vinifera hairy root line was used for its ability to produce several stilbenes, including trans-resveratrol, trans-piceatannol, cis-vitisin B, trans-piceid, pallidol, and trans-ε-viniferin. The influence of methyl-β-cyclodextrin (βCD) and Linecaps® maltodextrin was assessed during the growth phase, prior to elicitation (induced after a complete medium renewal). Stilbenes were extracted 7 days after elicitation from both the culture medium and the roots as crude extracts. Their antioxidant activities were evaluated using four in vitro assays (ABTS, DPPH, FRAP and CUPRAC) and their antimicrobial activities were tested on three skin-associated bacteria (Acinetobacter calcoaceticus, Corynebacterium xerosis, Staphylococcus epidermis). Cytotoxicity was assessed using an immortalized human keratinocyte line (HaCaT). Correlations between stilbenes content and bioactivity were examined. Roots pre-treated with βCD produced significantly more stilbenes (27,5 mg/100 mg crude extract) compared to positive control (10,3 mg/100 mg crude extract) and increased dimer formation (8 mg Viniferin/100 mg crude extract against 1,5 mg Viniferin/100 mg crude extract in positive control), suggesting a potentializing effect. In contrast, Linecaps-treated roots produced similar stilbenes levels than the positive control but showed a significant increase in roots biomass (1,5 g of fresh biomass against 0,6 g in positive control condition). Crude extracts from the culture medium cultured with βCD, rich in secreted stilbenes, exhibited the strongest antioxidant (ABTS: 0,5 g Trolox / g extract; CUPRAC: 0,3 g Trolox / g extract) and antimicrobial effects (IC50 = 0,310 mg.mL− 1 for Staphylococcus epidermis), with similar cytotoxicity to less enriched extracts (IC50 = 0,864 mg.mL− 1 compared to pure resveratrol which have an IC50 = 0,099 mg.mL− 1). This hairy root system proves to be a promising platform for producing bioactive plant compounds with potential cosmetic applications.