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<p>The tropical forest tree, <i>Dipterocarpus alatus</i> Roxb. is a potential source of valuable bioactive compounds, such as α-tocopherol, eburnamonine and lignin. Thus, calli were first induced using varying concentrations of thidiazuron (TDZ) in combination with 2,4-D and BA, after which the presence of bioactive compounds was investigated. This experiment employed completely randomized design with 4 replications. Shoot tip and young stem explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5 mg/l TDZ, 1.0–2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), and 5.0 mg/l benzyladenine (BA) and 500 mg/l polyvinylpyrrolidone (PVP) was added to the all treatments for 6 weeks. The results showed that MS mediun containing 0.5 mg/l TDZ + 1.0 mg/l 2,4-D (T1) was the most effective for callus induction derived from both types of explants, indicated by increased callus fresh weight (FW), and a healthy green appearance. Conversely, MS medium containing 0.5 mg/l TDZ + 1.0 mg/l 2,4-D + 5.0 mg/l BA (T2) and 0.5 mg/l TDZ + 2.0 mg/l 2,4-D + 5.0 mg/l BA (T3) inhibited calli formation by reducing FW and causing browning. Additionally, 8-bioactive compounds were identified from callus cultured on T1 medium. 2,2-Dihydroperoxypropane and 1-methylurate were the most bioactive compounds extracted from shoot tips and young stem calli, respectively. These findings suggested that a balance of 1.0 mg/l 2,4-D combined with 0.5 mg/l TDZ promoted efficient callus induction and provided bioactive compound production under in vitro conditions. Further study is required to characterize these bioactive compounds and to upscale production strategies.</p>

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Optimization of callus induction and bioactive compound production in Dipterocarpus alatus Roxb. using auxin–cytokinin combinations

  • 期刊论文

  • 发表日期：

    2025年12月3日

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  • Worodiyanti Tri Hapsari,
  • Thitiporn Pithayawutwinit,
  • Kritsada Burakri,
  • Patamaporn Tilarux,
  • Pranee Chaiyakorn,
  • Aranya Pimmongkol,
  • Anoma Dongsansuk

摘要

The tropical forest tree, Dipterocarpus alatus Roxb. is a potential source of valuable bioactive compounds, such as α-tocopherol, eburnamonine and lignin. Thus, calli were first induced using varying concentrations of thidiazuron (TDZ) in combination with 2,4-D and BA, after which the presence of bioactive compounds was investigated. This experiment employed completely randomized design with 4 replications. Shoot tip and young stem explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5 mg/l TDZ, 1.0–2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), and 5.0 mg/l benzyladenine (BA) and 500 mg/l polyvinylpyrrolidone (PVP) was added to the all treatments for 6 weeks. The results showed that MS mediun containing 0.5 mg/l TDZ + 1.0 mg/l 2,4-D (T1) was the most effective for callus induction derived from both types of explants, indicated by increased callus fresh weight (FW), and a healthy green appearance. Conversely, MS medium containing 0.5 mg/l TDZ + 1.0 mg/l 2,4-D + 5.0 mg/l BA (T2) and 0.5 mg/l TDZ + 2.0 mg/l 2,4-D + 5.0 mg/l BA (T3) inhibited calli formation by reducing FW and causing browning. Additionally, 8-bioactive compounds were identified from callus cultured on T1 medium. 2,2-Dihydroperoxypropane and 1-methylurate were the most bioactive compounds extracted from shoot tips and young stem calli, respectively. These findings suggested that a balance of 1.0 mg/l 2,4-D combined with 0.5 mg/l TDZ promoted efficient callus induction and provided bioactive compound production under in vitro conditions. Further study is required to characterize these bioactive compounds and to upscale production strategies.