Application of chlorine dioxide to enhance efficient bacteria elimination of contaminated shoot and its proliferation of Alocasia melo
摘要
Contamination limits in vitro plant regeneration caused to low multiplication rate and plant death. Disinfestation of contaminated shoot by chlorine dioxide (ClO2) can overcome those problems and also reduce time consuming process, energy and loss of nutrient available from autoclaving. So, the objectives of this research were to study effects of ClO2 on bacteria elimination of contaminated shoot and to investigate its effect on growth of Alocasia melo along with root induction and acclimatization. The bacteria-contaminated shoots were cultured on different concentrations of ClO2 for 4 weeks. The results found that 100 mg/L ClO2 containing shoot proliferation medium gave 100% disinfestation, survival rate and shoot proliferation and also promoted plant growth. For bacteria quantification and identification, endogenous bacteria isolated from plant were higher than those from culture medium. Two groups of bacteria were found; Clostridium sp. and Bacillus sp. For root induction, 40 mg/L ClO2 containing root induction medium gave the best results in disinfestation and root induction at 100%. However, there were not significant differences in acclimatization among plantlets from 20 to 40 mg/L ClO2 containing medium. Therefore, our results indicate that ClO2 can eliminate shoot-contaminated bacteria and improve growth of in vitro plant without autoclaving that is a new finding for mass propagation.