Scholarlink

<p>Enhancing the horticultural and commercial value of <i>Ananas comosus</i> through polyploidy has great potential. This study used different colchicine treatments, durations, and concentrations to induce polyploidy in pineapple embryogenic callus. A maximum polyploid induction rate of 40% was achieved in embryogenic callus exposed to 0.1% colchicine via co-cultivation for 20 days. Flow cytometry revealed 202 tetraploids among the regenerated plants analyzed. Polyploid plants, in contrast to diploid ones, are morphologically characterized by having thicker, heavier, wider, and shorter leaves, a larger leaf area, a darker color, a shorter overall height, thicker stems, and fewer, shorter leaf spines that are more irregularly distributed and differently shaped. Polyploid plants differ from diploids in terms of stomatal and cellular traits: their stomata are larger but less abundant, while the stomatal guard cells and leaf epidermal cells are also larger in dimension. The study revealed that polyploid plants exhibited much higher levels of chlorophyll and PEPC enzyme activity than the diploid control plants. Compared to diploid control plants, polyploid plants exhibit enhanced tolerance to salinity and heat stress. The present study outlining the induction of polyploids in pineapple broaden the germplasm for future improvement of the crop.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Analysis of colchicine-induced polyploidy in pineapple (Ananas comosus L.)

  • 期刊论文

  • 发表日期：

    2025年11月17日

查看全文
  • Junshuo Qiu,
  • Yuanyuan Wang,
  • Jing Zhang,
  • Tao Li,
  • Yuanrong Xu,
  • Tingting Wang,
  • Tao Xie

摘要

Enhancing the horticultural and commercial value of Ananas comosus through polyploidy has great potential. This study used different colchicine treatments, durations, and concentrations to induce polyploidy in pineapple embryogenic callus. A maximum polyploid induction rate of 40% was achieved in embryogenic callus exposed to 0.1% colchicine via co-cultivation for 20 days. Flow cytometry revealed 202 tetraploids among the regenerated plants analyzed. Polyploid plants, in contrast to diploid ones, are morphologically characterized by having thicker, heavier, wider, and shorter leaves, a larger leaf area, a darker color, a shorter overall height, thicker stems, and fewer, shorter leaf spines that are more irregularly distributed and differently shaped. Polyploid plants differ from diploids in terms of stomatal and cellular traits: their stomata are larger but less abundant, while the stomatal guard cells and leaf epidermal cells are also larger in dimension. The study revealed that polyploid plants exhibited much higher levels of chlorophyll and PEPC enzyme activity than the diploid control plants. Compared to diploid control plants, polyploid plants exhibit enhanced tolerance to salinity and heat stress. The present study outlining the induction of polyploids in pineapple broaden the germplasm for future improvement of the crop.