Scholarlink

<p><i>Salix brachista</i>, commonly known as Cushion willow, is a common component of alpine subnival assemblages in the Hengduan Mountains and adjacent areas. It has a creeping stem with height of no more than 5&#xa0;cm, which is likely an adaptation to its harsh environmental conditions such as strong winds, high solar radiation, and drastic daily temperature fluctuations. As a typical alpine woody plant, the development of a genetic transformation system for <i>Salix brachista</i> is valuable for unraveling genetic basis of plant alpine adaptive evolution and for mining alpine stress-resistant gene resources. In the course of our research, we initially focused on creating a robust regeneration system for this species, utilizing stem segments of <i>Salix brachista</i>. Through meticulous optimization of various factors, we achieved a commendable average regeneration rate of 40.0%. Building upon this foundational work, we then established a straightforward and highly effective genetic transformation protocol, employing the <i>Agrobacterium</i>-mediated method. The successful establishment of this genetic transformation method not only represents a significant advancement in itself but also opens up new avenues for further gene editing and functional research endeavors in <i>Salix brachista</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Establishment of Agrobacterium-mediated genetic transformation in Salix brachista

  • 期刊论文

  • 发表日期：

    2025年11月5日

查看全文
  • Dan Tang,
  • Yumeng Li,
  • García-Caparrós Pedro,
  • Jiahui Chen,
  • Xudong Sun

摘要

Salix brachista, commonly known as Cushion willow, is a common component of alpine subnival assemblages in the Hengduan Mountains and adjacent areas. It has a creeping stem with height of no more than 5 cm, which is likely an adaptation to its harsh environmental conditions such as strong winds, high solar radiation, and drastic daily temperature fluctuations. As a typical alpine woody plant, the development of a genetic transformation system for Salix brachista is valuable for unraveling genetic basis of plant alpine adaptive evolution and for mining alpine stress-resistant gene resources. In the course of our research, we initially focused on creating a robust regeneration system for this species, utilizing stem segments of Salix brachista. Through meticulous optimization of various factors, we achieved a commendable average regeneration rate of 40.0%. Building upon this foundational work, we then established a straightforward and highly effective genetic transformation protocol, employing the Agrobacterium-mediated method. The successful establishment of this genetic transformation method not only represents a significant advancement in itself but also opens up new avenues for further gene editing and functional research endeavors in Salix brachista.