Scholarlink

<p><i>Astragalus membranaceus</i> (Fisch.) Bunge (<i>A</i>. <i>membranaceus</i>) is a pharmacologically important herb, yet its traditional cultivation and slow growth cycle limit supply. Callus induction represents an effective biotechnological approach to enhance bioactive compound production through in vitroculture. This study investigated chitosan (CHT: 50, 100, 150, 200&#xa0;mg/L) and brassinolide (BL: 0.01, 0.05, 0.1, 0.15&#xa0;mg/L) as elicitors for enhancing callus growth, antioxidant capacity, and secondary metabolite accumulation. All treatments significantly improved physiological traits, antioxidant parameters, and bioactive compound accumulation, with effects dependent on elicitor type and concentration. Treatment with 200&#xa0;mg/L CHT maximized FW (+ 49.52%) and superoxide dismutase (SOD) activity (+ 108.33%), while suppressing malondialdehyde (MDA) content (-96.76%) and superoxide anion (O<sub>2</sub>·<sup>−</sup>) generation (-68.01%). It simultaneously induced the highest accumulation of alkaloids (6.39-fold), total flavonoids (2.99-fold), and total saponins (2.63-fold) compared to controls. Maximum polysaccharide content (+ 50.97%) was achieved at 50&#xa0;mg/L CHT. The 0.1&#xa0;mg/L BL treatment optimized dry weight (+ 88.10%), proline (+ 250%), glutathione (+ 151%), and SOD activity (+ 216.67%), while minimizing MDA (-95.52%) and O<sub>2</sub>·<sup>−</sup> (-61.63%) accumulation. This treatment also elevated flavonoid (3.55-fold), saponin (1.86-fold), and polysaccharide (+ 51.19%) content, and enhanced phenylalanine ammonia-lyase activity. These findings establish a foundation for scaling callus culture and optimizing pharmaceutical compound production, enabling concentration-specific optimization for industrial applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effects of brassinolide and chitosan on the growth and medicinal value of Astragalus membranaceus callus

  • 期刊论文

  • 发表日期：

    2025年11月17日

查看全文
  • Chengdan Shi,
  • Liuqian Chen,
  • Ziyuan Yan,
  • Boda Wang,
  • Junyan Hu,
  • Guixia Liu

摘要

Astragalus membranaceus (Fisch.) Bunge (A. membranaceus) is a pharmacologically important herb, yet its traditional cultivation and slow growth cycle limit supply. Callus induction represents an effective biotechnological approach to enhance bioactive compound production through in vitroculture. This study investigated chitosan (CHT: 50, 100, 150, 200 mg/L) and brassinolide (BL: 0.01, 0.05, 0.1, 0.15 mg/L) as elicitors for enhancing callus growth, antioxidant capacity, and secondary metabolite accumulation. All treatments significantly improved physiological traits, antioxidant parameters, and bioactive compound accumulation, with effects dependent on elicitor type and concentration. Treatment with 200 mg/L CHT maximized FW (+ 49.52%) and superoxide dismutase (SOD) activity (+ 108.33%), while suppressing malondialdehyde (MDA) content (-96.76%) and superoxide anion (O2·−) generation (-68.01%). It simultaneously induced the highest accumulation of alkaloids (6.39-fold), total flavonoids (2.99-fold), and total saponins (2.63-fold) compared to controls. Maximum polysaccharide content (+ 50.97%) was achieved at 50 mg/L CHT. The 0.1 mg/L BL treatment optimized dry weight (+ 88.10%), proline (+ 250%), glutathione (+ 151%), and SOD activity (+ 216.67%), while minimizing MDA (-95.52%) and O2·− (-61.63%) accumulation. This treatment also elevated flavonoid (3.55-fold), saponin (1.86-fold), and polysaccharide (+ 51.19%) content, and enhanced phenylalanine ammonia-lyase activity. These findings establish a foundation for scaling callus culture and optimizing pharmaceutical compound production, enabling concentration-specific optimization for industrial applications.