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<p><i>Celastrus paniculatus</i> (Willd.), often referred as “Intellect Tree”, is a medicinally significant climber used in Ayurvedic medicine for managing various cognitive functions and neurological disorders. Celastrol, a triterpenoid quinone methide, has garnered considerable attention because of its neuroprotective, anti-inflammatory, antioxidant, and anti-cancerous properties. The availability of this valuable plant is insufficient to fulfill growing demands, because of the paucity of research and overharvesting of wild populations. Present study offers a sustainable method for large scale production of celastrol by presenting, for the first time, a methodology for root derived suspension culture of <i>C. paniculatus</i>. Maximum callus induction frequency i.e. 80.50% was achieved on Murashige and Skoog (MS) medium containing 5.0 µM thidiazuron (TDZ) + 1.0 µM Indole butyric acid (IBA). Chitosan (CH) and yeast extract (YE), two biotic elicitors, were investigated at doses of 50, 100, 200, and 300 µM during a range of contact times (24, 48, 72, and 96&#xa0;h). Chitosan proved more effective than YE, with the highest biomass accumulation (73.2 ± 0.56&#xa0;g/L FW; 8.8 ± 0.32&#xa0;g/L DW) with celastrol content (1.25&#xa0;mg/g) achieved at 200 µM CH after 72&#xa0;h, resulting in 1.08-fold increase in comparison to control (1.15&#xa0;mg/g). These results highlight the potential of elicitor-based suspension cultures for the eco-friendly enhancement of celastrol production, offering a sustainable and eco-friendly approach for commercial-scale production of bioactive compounds from endangered medicinal plants.</p>

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Comparative elicitation efficiency of chitosan and yeast extract for enhanced production of celastrol in root derived callus of Celastrus paniculatus (Willd.)

  • 期刊论文

  • 发表日期：

    2025年10月30日

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  • Sabaha Tahseen,
  • Anwar Shahzad,
  • Firdaus Qamar,
  • Adla Wasi,
  • Irfan Bashir Ganie

摘要

Celastrus paniculatus (Willd.), often referred as “Intellect Tree”, is a medicinally significant climber used in Ayurvedic medicine for managing various cognitive functions and neurological disorders. Celastrol, a triterpenoid quinone methide, has garnered considerable attention because of its neuroprotective, anti-inflammatory, antioxidant, and anti-cancerous properties. The availability of this valuable plant is insufficient to fulfill growing demands, because of the paucity of research and overharvesting of wild populations. Present study offers a sustainable method for large scale production of celastrol by presenting, for the first time, a methodology for root derived suspension culture of C. paniculatus. Maximum callus induction frequency i.e. 80.50% was achieved on Murashige and Skoog (MS) medium containing 5.0 µM thidiazuron (TDZ) + 1.0 µM Indole butyric acid (IBA). Chitosan (CH) and yeast extract (YE), two biotic elicitors, were investigated at doses of 50, 100, 200, and 300 µM during a range of contact times (24, 48, 72, and 96 h). Chitosan proved more effective than YE, with the highest biomass accumulation (73.2 ± 0.56 g/L FW; 8.8 ± 0.32 g/L DW) with celastrol content (1.25 mg/g) achieved at 200 µM CH after 72 h, resulting in 1.08-fold increase in comparison to control (1.15 mg/g). These results highlight the potential of elicitor-based suspension cultures for the eco-friendly enhancement of celastrol production, offering a sustainable and eco-friendly approach for commercial-scale production of bioactive compounds from endangered medicinal plants.