Influence of sodium selenite concentrations on growth and selenium accumulation in tissue-cultured seedlings of Trichosanthes kirilowii Maxim

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发表日期：

2025年10月22日

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Lai Xiyue,
Muhammad Ahmad Hassan,
Zhong Baishuo,
Cui Guangrong,
He Keqin

摘要

Trichosanthes kirilowii Maxim is a medicinal herb of the gourd family and has multiple health benefits. Root propagation is a standard breeding method for Trichosanthes, but root propagation can easily cause the spread of diseases and pests in the soil. Tissue culture is a modern technique that rapidly reproduces it, and the regenerated seedlings are virus-free. This research study explored the effect of different concentrations of sodium selenite (Se1: 0.00 mg L^− 1, Se2: 0.0005 mg L^− 1, Se3: 0.001 mg L^− 1, Se4: 0.005 mg L^− 1, Se5: 0.01 mg L^− 1, Se6: 0.05 mg L^− 1, Se7: 0.10 mg L^− 1, Se8: 0.20 mg L^− 1, Se9: 0.40 mg L^− 1, Se10: 0.60 mg L^− 1, Se11: 0.80 mg L^− 1, Se12: 1.00 mg L^− 1) on the tissue-cultured regenerated seedlings of T. kirilowii Maxim. Using aseptic seedlings of T. kirilowii Maxim as materials, sodium selenite was added to the rooting medium. It analyzed the morpho-physiological and biochemical indices, and the distribution of selenium content-enrichment in various plant parts (leaf, stem, root) with comparison to the control (Se1). When the concentration of sodium selenite was 0.005 mg L^− 1 (Se4), it improved the morphological traits such as root activity, root growth, plant height, leaf area and dry matter accumulation and physiological traits such as photosynthetic pigment contents, soluble protein contents of regenerated seedlings of T. kirilowii. Upon 0.05 mg L^− 1 (Se6), it increased the soluble sugar and reduced the sugar contents in regenerated seedlings; with the increase in sodium selenite concentration, the total selenium and organic-selenium contents gradually increased in the roots, stems, and leaves. Meanwhile, the conversion rate of organic selenium was the highest in leaves, followed by stems and roots, respectively. The results suggested that the appropriate concentration of sodium selenite can promote the growth of T. kirilowii Maxim. This research investigation provided a reference for the production and breeding of selenium-rich T. kirilowii Maxim.