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<p>Drought stress has a severe impact on plant growth and physiological activities. In recent years, extensive research has been conducted on how plants adapt to drought through various mechanisms, including morphological, physiological, and molecular adaptations. Among these, the WRKY transcription factor family plays a key role in the response of plants to abiotic stress. This study analyzed the expression pattern and the role of <i>PuWRKY21</i> under drought stress. The current study found that the expression of <i>PuWRKY21</i> in roots and leaves was significantly increased under drought (PEG 6000) and ABA stress conditions, particularly in roots. It showed that under normal conditions, the number of adventitious roots and lateral roots in <i>PuWRKY21-OE</i> transgenic lines was similar to that of the wild type (WT), while the <i>PuWRKY21-IE</i> transgenic lines exhibited more than the WT. Under drought stress, the lateral roots of <i>PuWRKY21-OE</i> lines hardly grew, and the length of adventitious roots showed no significant increase. In contrast, the <i>PuWRKY21-IE</i> transgenic lines displayed significantly greater length and number of adventitious roots compared to both WT and <i>PuWRKY21-OE</i> lines, with more developed lateral roots and growing well. In summary, the inhibiting expression of <i>PuWRKY21</i> can enhance poplar drought resistance. The research revealed a new function of <i>WRKY21</i> transcription factors in woody plant stress resistance, which expanding the molecular network understanding of plant stress responses, and providing a theoretical basis for breeding new drought-resistant poplar varieties.</p>

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PuWRKY21 is crucial as a negative regulatory factor in response to drought stress in poplar

  • 期刊论文

  • 发表日期：

    2025年10月13日

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  • Zihan Fan,
  • Hui Li,
  • Wanxin Li,
  • Jingli Yang

摘要

Drought stress has a severe impact on plant growth and physiological activities. In recent years, extensive research has been conducted on how plants adapt to drought through various mechanisms, including morphological, physiological, and molecular adaptations. Among these, the WRKY transcription factor family plays a key role in the response of plants to abiotic stress. This study analyzed the expression pattern and the role of PuWRKY21 under drought stress. The current study found that the expression of PuWRKY21 in roots and leaves was significantly increased under drought (PEG 6000) and ABA stress conditions, particularly in roots. It showed that under normal conditions, the number of adventitious roots and lateral roots in PuWRKY21-OE transgenic lines was similar to that of the wild type (WT), while the PuWRKY21-IE transgenic lines exhibited more than the WT. Under drought stress, the lateral roots of PuWRKY21-OE lines hardly grew, and the length of adventitious roots showed no significant increase. In contrast, the PuWRKY21-IE transgenic lines displayed significantly greater length and number of adventitious roots compared to both WT and PuWRKY21-OE lines, with more developed lateral roots and growing well. In summary, the inhibiting expression of PuWRKY21 can enhance poplar drought resistance. The research revealed a new function of WRKY21 transcription factors in woody plant stress resistance, which expanding the molecular network understanding of plant stress responses, and providing a theoretical basis for breeding new drought-resistant poplar varieties.