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<p>VQ proteins are plant-specific regulators with a conserved valine-glutamine (VQ) motif that modulate growth, development and stress responses. However, the biological roles of VQ genes in cucumber remain unclear. In this study, a cucumber VQ family gene member <i>CsVQ12</i> was first cloned for functional verification. This gene was induced by methyl jasmonate (MeJA) treatment, and CsVQ12 functions as a transcriptional activator localized in the nucleus. Compared with wild-type (WT) plants under freezing stress, <i>CsVQ12</i>-transgenic <i>Arabidopsis</i> plants exhibited lower malondialdehyde (MDA) content and relative electrolyte leakage, along with higher peroxidase (POD) activity and upregulated expression of endogenous cold-response genes (<i>AtCBF2</i>, <i>AtCBF3</i>, <i>AtCOR15A</i>, and <i>AtKIN1</i>), which might enhance stress tolerance. Furthermore, <i>CsVQ12</i> overexpression promoted leaf senescence in <i>Arabidopsis</i>, accompanied by altered expression of senescence-associated genes. Comprehensive phenotypic and physiological analyses confirmed that <i>CsVQ12</i> likely regulates both leaf senescence and freezing stress tolerance in cucumber, potentially through the jasmonic acid (JA) signaling pathway. These findings establish a basis for understanding <i>CsVQ12</i> function and provide valuable genetic targets for developing early-maturing and freezing-resistant cucumber varieties.</p>

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Overexpression of cucumber CsVQ12 gene modulates MeJA-mediated freezing tolerance and leaf senescence in Arabidopsis

  • 期刊论文

  • 发表日期：

    2025年9月29日

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  • Feng Yang,
  • Hailin Xia,
  • Jindong Zhao,
  • Xueping Guo,
  • Meng Wang,
  • Ziyu Lin,
  • Dongdong Mao,
  • Zhaoyang Hu,
  • Yuelong Zhou,
  • Shiqiang Liu,
  • Yong Zhou

摘要

VQ proteins are plant-specific regulators with a conserved valine-glutamine (VQ) motif that modulate growth, development and stress responses. However, the biological roles of VQ genes in cucumber remain unclear. In this study, a cucumber VQ family gene member CsVQ12 was first cloned for functional verification. This gene was induced by methyl jasmonate (MeJA) treatment, and CsVQ12 functions as a transcriptional activator localized in the nucleus. Compared with wild-type (WT) plants under freezing stress, CsVQ12-transgenic Arabidopsis plants exhibited lower malondialdehyde (MDA) content and relative electrolyte leakage, along with higher peroxidase (POD) activity and upregulated expression of endogenous cold-response genes (AtCBF2, AtCBF3, AtCOR15A, and AtKIN1), which might enhance stress tolerance. Furthermore, CsVQ12 overexpression promoted leaf senescence in Arabidopsis, accompanied by altered expression of senescence-associated genes. Comprehensive phenotypic and physiological analyses confirmed that CsVQ12 likely regulates both leaf senescence and freezing stress tolerance in cucumber, potentially through the jasmonic acid (JA) signaling pathway. These findings establish a basis for understanding CsVQ12 function and provide valuable genetic targets for developing early-maturing and freezing-resistant cucumber varieties.