Scholarlink

<p>Chalcone synthase (CHS) genes are known to diversify into a super-gene family in plants. In our previous study, <i>DchCHS1</i> (KX893854) and <i>DchCHS3</i> (MK416198) are related to flower colors and the former plays a dominant role in <i>Dianthus chinensis</i>. Their deduced amino acids have a close relation with the known CHS sequences in Caryophyllaceae. To further compare the function of <i>DchCHS1</i> and <i>DchCHS3</i>, we analyzed their subcellular localization, prokaryotic expression and overexpression in tobacco. It was found that both were expressed in the cytoplasm and nucleus, and had the CHS enzyme activity by catalyzing <i>p</i>-coumaroyl-CoA and malonyl-CoA to naringin chalcones. The molecular weight of DchCHS1 was smaller than that of DchCHS3. Compared to the wild type, <i>DchCHSs</i>-overexpressing lines dramatically displayed thinner leaves, lower photosynthetic rates, reduced chlorophyll contents, and elevated antioxidant enzyme activities. MRNA-seq analysis of tobacco leaves revealed that upregulated DEGs in <i>DchCHSs</i>-OE lines were significantly enriched in the flavonoid pathway, while downregulated DEGs were significantly enriched in the photosynthesis pathway. The significant elevation of antioxidant enzyme activities and enrichment of flavonoid-related DEGs suggest that <i>DchCHS1</i> and <i>DchCHS3</i> might enhance resistance in <i>DchCHSs</i>-OE lines. <i>DchCHSs</i>-OE lines flowers exhibited significant increase in contents of anthocyanin and flavonoid compared to WT, accompanied by visibly deeper pigmentation. Compared to <i>DchCHS1</i>-OE lines, <i>DchCHS3</i>-OE lines obviously displayed thicker leaves, lower photosynthetic rates and chlorophyll content, higher antioxidant enzyme activity, as well as lighter flower color and decreased anthocyanin content in petals. The results indicate that <i>DchCHS1</i> and <i>DchCHS3</i> enhance flower colors and might improve resistance in plants, and <i>DchCHS1</i> is much more important in flower colors.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Subcellular localization and prokaryotic expression of DchCHS1 and DchCHS3 from Dianthus chinensis, and their overexpression in tobacco

  • 期刊论文

  • 发表日期：

    2025年9月11日

查看全文
  • Sheng-Yan Hu,
  • Hui Liu,
  • Xue-Qin He

摘要

Chalcone synthase (CHS) genes are known to diversify into a super-gene family in plants. In our previous study, DchCHS1 (KX893854) and DchCHS3 (MK416198) are related to flower colors and the former plays a dominant role in Dianthus chinensis. Their deduced amino acids have a close relation with the known CHS sequences in Caryophyllaceae. To further compare the function of DchCHS1 and DchCHS3, we analyzed their subcellular localization, prokaryotic expression and overexpression in tobacco. It was found that both were expressed in the cytoplasm and nucleus, and had the CHS enzyme activity by catalyzing p-coumaroyl-CoA and malonyl-CoA to naringin chalcones. The molecular weight of DchCHS1 was smaller than that of DchCHS3. Compared to the wild type, DchCHSs-overexpressing lines dramatically displayed thinner leaves, lower photosynthetic rates, reduced chlorophyll contents, and elevated antioxidant enzyme activities. MRNA-seq analysis of tobacco leaves revealed that upregulated DEGs in DchCHSs-OE lines were significantly enriched in the flavonoid pathway, while downregulated DEGs were significantly enriched in the photosynthesis pathway. The significant elevation of antioxidant enzyme activities and enrichment of flavonoid-related DEGs suggest that DchCHS1 and DchCHS3 might enhance resistance in DchCHSs-OE lines. DchCHSs-OE lines flowers exhibited significant increase in contents of anthocyanin and flavonoid compared to WT, accompanied by visibly deeper pigmentation. Compared to DchCHS1-OE lines, DchCHS3-OE lines obviously displayed thicker leaves, lower photosynthetic rates and chlorophyll content, higher antioxidant enzyme activity, as well as lighter flower color and decreased anthocyanin content in petals. The results indicate that DchCHS1 and DchCHS3 enhance flower colors and might improve resistance in plants, and DchCHS1 is much more important in flower colors.