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<p>The production of haploid and doubled haploid (DH) plant is essential in accelerating the development of pure lines in crop breeding. Despite the availability of various in vitro and in vivo methods for haploid plant production, these technologies are often restricted to a limited range of genotypes across many plant species. In melon, parthenogenesis is commonly employed technique for generating haploid plants. This study aimed to evaluate the efficiency of parthenogenesis in <i>Cucumis melo</i>, inodorus group ‘Khatooni’ for haploid plant production. We investigated several critical aspects of the parthenogenesis process”, including fruit set following pollination with gamma irradiated pollens (IPs), pollen culture, production of haploid embryos, the in vitro germination and plant regeneration of parthenogenic embryos, along with techniques for chromosome doubling. Furthermore, we assessed the parthenogenesis potential of the Khatooni cultivar to optimize the entire process. Our results demonstrated successful parthenogenesis in this cultivar, yielding 22 parthenogenic fruits from 132 crosses. Among these fruits, we rescued 110 parthenogenic embryos at various developmental stages, resulting in the production of 53 plantlets. Flow cytometry (FC) analysis revealed that 48 of these plantlets were haploid.</p>

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Parthenogenesis-based production of doubled haploids in melon (Cucumis melo L.)

  • 期刊论文

  • 发表日期：

    2025年9月11日

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  • Vali Farzifard-Kambelash,
  • Ahmad Moieni,
  • Raheleh Karimi-Ashtiyani,
  • Mohammad Sadegh Sabet

摘要

The production of haploid and doubled haploid (DH) plant is essential in accelerating the development of pure lines in crop breeding. Despite the availability of various in vitro and in vivo methods for haploid plant production, these technologies are often restricted to a limited range of genotypes across many plant species. In melon, parthenogenesis is commonly employed technique for generating haploid plants. This study aimed to evaluate the efficiency of parthenogenesis in Cucumis melo, inodorus group ‘Khatooni’ for haploid plant production. We investigated several critical aspects of the parthenogenesis process”, including fruit set following pollination with gamma irradiated pollens (IPs), pollen culture, production of haploid embryos, the in vitro germination and plant regeneration of parthenogenic embryos, along with techniques for chromosome doubling. Furthermore, we assessed the parthenogenesis potential of the Khatooni cultivar to optimize the entire process. Our results demonstrated successful parthenogenesis in this cultivar, yielding 22 parthenogenic fruits from 132 crosses. Among these fruits, we rescued 110 parthenogenic embryos at various developmental stages, resulting in the production of 53 plantlets. Flow cytometry (FC) analysis revealed that 48 of these plantlets were haploid.