Scholarlink

<p><i>Anoectochilus lylei</i>, a valuable medicinal orchid, is recognized for its rich profile of pharmacologically active compounds. This study investigated strategies to enhance the accumulation of bioactive metabolites in <i>A. lylei</i> rhizome cultures under different elicitor treatments and bioreactor conditions. Treatments with methyl jasmonate (MeJA) at various concentrations had no significant impact on rhizome biomass parameters, including fresh weight (FW), dry weight (DW), or growth index, but significantly elevated kinsenoside levels at 100 and 400 µM (5.7–5.9&#xa0;mg/g DW), approximately 2.5-fold higher than the control. Total phenolic and flavonoid contents remained largely unchanged across MeJA treatments, while polysaccharide levels declined with increasing MeJA concentration. In contrast, salicylic acid (SA) elicitation, particularly at 200 µM, led to a pronounced increase in kinsenoside content (26.78&#xa0;mg/g DW), representing an 11-fold enhancement over the control, without significantly affecting biomass parameters. SA also moderately influenced the accumulation of phenolics, flavonoids, and polysaccharides. Bioreactor cultivation using varying inoculum densities revealed that higher densities (up to 45&#xa0;g/L) significantly increased FW and DW, while growth efficiency and percentage DW remained stable. Maximum kinsenoside accumulation occurred at 30&#xa0;g/L inoculum, while total phenolics and flavonoids contents were enhanced at 15 and 45&#xa0;g/L; however, polysaccharide content declined with increased inoculum density. Moreover, extracts from SA- and MeJA-induced rhizomes exhibited strong antibacterial activity against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>, with minimum inhibitory concentrations (MIC) values of 6.25&#xa0;mg/mL. Bioreactor-derived rhizome extract also demonstrated antibacterial effects. These findings highlight the potential of elicitation and bioreactor-based strategies for scalable production of bioactive-rich <i>A. lylei</i> rhizomes with promising antibacterial properties.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Elicitation and bioreactor-based strategies to enhance bioactive compound accumulation and antibacterial activity in Anoectochilus Lylei rhizome cultures

  • 期刊论文

  • 发表日期：

    2025年9月12日

查看全文
  • My Khanh Tran Thi Ha,
  • Thanh-Tam Ho,
  • Thi Xuan Tuy Ho,
  • Tolulope Joshua Ashaolu,
  • Gia Huy Ngo,
  • Trung Hieu Le,
  • Van Ngo Thai Bich,
  • Trung Thanh Nguyen,
  • Hoang Thi Kim Hong,
  • Thanh-Do Le,
  • So-Young Park

摘要

Anoectochilus lylei, a valuable medicinal orchid, is recognized for its rich profile of pharmacologically active compounds. This study investigated strategies to enhance the accumulation of bioactive metabolites in A. lylei rhizome cultures under different elicitor treatments and bioreactor conditions. Treatments with methyl jasmonate (MeJA) at various concentrations had no significant impact on rhizome biomass parameters, including fresh weight (FW), dry weight (DW), or growth index, but significantly elevated kinsenoside levels at 100 and 400 µM (5.7–5.9 mg/g DW), approximately 2.5-fold higher than the control. Total phenolic and flavonoid contents remained largely unchanged across MeJA treatments, while polysaccharide levels declined with increasing MeJA concentration. In contrast, salicylic acid (SA) elicitation, particularly at 200 µM, led to a pronounced increase in kinsenoside content (26.78 mg/g DW), representing an 11-fold enhancement over the control, without significantly affecting biomass parameters. SA also moderately influenced the accumulation of phenolics, flavonoids, and polysaccharides. Bioreactor cultivation using varying inoculum densities revealed that higher densities (up to 45 g/L) significantly increased FW and DW, while growth efficiency and percentage DW remained stable. Maximum kinsenoside accumulation occurred at 30 g/L inoculum, while total phenolics and flavonoids contents were enhanced at 15 and 45 g/L; however, polysaccharide content declined with increased inoculum density. Moreover, extracts from SA- and MeJA-induced rhizomes exhibited strong antibacterial activity against Staphylococcus aureus and Escherichia coli, with minimum inhibitory concentrations (MIC) values of 6.25 mg/mL. Bioreactor-derived rhizome extract also demonstrated antibacterial effects. These findings highlight the potential of elicitation and bioreactor-based strategies for scalable production of bioactive-rich A. lylei rhizomes with promising antibacterial properties.