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<p><i>Petunia hybrida</i> is a commonly used horticultural ornamental plant. At present, there is no genome-wide identification and analysis of the cyclic nucleotide-gated channel (CNGC) gene family in Petunia. In this study, we identified the CNGC gene family in petunia, and the phylogenetic results divided 16 <i>PhCNGCs</i> into four groups. Gene structure and motif analyses suggest that PhCNGC family proteins may have shown a clear preference during the evolutionary process. In addition, our study revealed the presence of many hormone, low temperature and drought response elements in the promoter region of the <i>PhCNGCs</i>, suggesting their important role in the regulation of environmental adaptation. Finally, we analyzed the expression patterns of all <i>PhCNGC</i> genes under cold stress, and found that <i>PhCNGC11</i> was significantly up-regulated under cold stress. Subsequently, the importance of this gene in the cold stress response of Petunia was initially verified by virus-induced gene silencing (VIGS) and the function of <i>PhCNGC11</i> gene was further verified by overexpression in yeast. In summary, our study provides a comprehensive understanding of the function and regulation mechanism of CNGC gene family in Petunia, which lays an important foundation for further research on the cold adaptability of Petunia, and also lays a theoretical foundation for the rational utilization of other related gene families in the genetic improvement of stress resistance of ornamental plants.</p>

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Genome-wide exploration of CNGC gene family in Petunia hybrida under cold stress, with functional characterization of PhCNGC11

  • 期刊论文

  • 发表日期：

    2025年9月11日

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  • Heng Zhang,
  • Ke Xu

摘要

Petunia hybrida is a commonly used horticultural ornamental plant. At present, there is no genome-wide identification and analysis of the cyclic nucleotide-gated channel (CNGC) gene family in Petunia. In this study, we identified the CNGC gene family in petunia, and the phylogenetic results divided 16 PhCNGCs into four groups. Gene structure and motif analyses suggest that PhCNGC family proteins may have shown a clear preference during the evolutionary process. In addition, our study revealed the presence of many hormone, low temperature and drought response elements in the promoter region of the PhCNGCs, suggesting their important role in the regulation of environmental adaptation. Finally, we analyzed the expression patterns of all PhCNGC genes under cold stress, and found that PhCNGC11 was significantly up-regulated under cold stress. Subsequently, the importance of this gene in the cold stress response of Petunia was initially verified by virus-induced gene silencing (VIGS) and the function of PhCNGC11 gene was further verified by overexpression in yeast. In summary, our study provides a comprehensive understanding of the function and regulation mechanism of CNGC gene family in Petunia, which lays an important foundation for further research on the cold adaptability of Petunia, and also lays a theoretical foundation for the rational utilization of other related gene families in the genetic improvement of stress resistance of ornamental plants.