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<p>An efficient in vitro regeneration protocol for <i>Incarvillea emodi</i> (Wallich ex Royle) Chatterjee, was developed using nodal explants. Meta-Topolin (mT) demonstrated superior performance in direct shoot organogenesis compared to Kinetin (Kn) and 6-benzylaminopurine (BAP). Optimal shoot organogenesis parameters, including the highest shoot regeneration rate of 93.33 ± 0.88% (%) with average number of shoots of 4.24 ± 0.76, having shoot length of 4.47 ± 0.84 centimeter (cm), were achieved using Murashige and Skoog (MS) media enriched with 1.0 mg L<sup>−1</sup> mT + 0.25 mg L<sup>−1</sup> α-Naphthalene acetic acid (NAA) following 6 weeks of culture. Enhanced shoot proliferation was observed on MS medium with 1.0 mg L<sup>−1</sup> mT + 0.25 mgL<sup>−1</sup> NAA + 0.30 mg L<sup>−1</sup> Gibberellic acid (GA<sub>3</sub>), yielding an average of 14.45 ± 0.57 shoots with an average length of 8.53 ± 1.78&#xa0;cm. Successful rooting at 94.73 ± 0.37% occurred on half-strength MS medium with 0.5 mg L<sup>−1</sup> Indole 3-butyric acid (IBA). In vitro raised plants showed ex vitro survival of 82.55% upon acclimatization. Biochemical analyses revealed that in vitro plants had higher levels of photopigments, phenolics, flavonoids, and antioxidant activity compared to donor plants. Gas chromatography-mass spectrometry (GC-MS) analysis indicated comparatively increased volatile phytochemical content in in vitro plants. Morphological and physiological assessments showed that in vitro plantlets developed larger, greener leaves, lower stomatal density, and higher photosynthetic efficiency than donor plants, supporting enhanced acclimatization and growth performance. This reproducible protocol provides a practical approach for the mass propagation, domestication, and commercial cultivation of <i>I. emodi</i>, facilitating its use as an ornamental and medicinal plant species.</p>

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A high-efficiency tissue culture protocol for the domestication and mass propagation of Incarvillea Emodi (Wallich ex Royle) chatterjee: a valuable wild ornamental and medicinal plant species

  • 期刊论文

  • 发表日期：

    2025年6月24日

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  • Vipasha Verma,
  • Priti,
  • Diksha Thakur,
  • Rohini Devi,
  • Kamlesh Verma,
  • Bhavya Bhargava

摘要

An efficient in vitro regeneration protocol for Incarvillea emodi (Wallich ex Royle) Chatterjee, was developed using nodal explants. Meta-Topolin (mT) demonstrated superior performance in direct shoot organogenesis compared to Kinetin (Kn) and 6-benzylaminopurine (BAP). Optimal shoot organogenesis parameters, including the highest shoot regeneration rate of 93.33 ± 0.88% (%) with average number of shoots of 4.24 ± 0.76, having shoot length of 4.47 ± 0.84 centimeter (cm), were achieved using Murashige and Skoog (MS) media enriched with 1.0 mg L−1 mT + 0.25 mg L−1 α-Naphthalene acetic acid (NAA) following 6 weeks of culture. Enhanced shoot proliferation was observed on MS medium with 1.0 mg L−1 mT + 0.25 mgL−1 NAA + 0.30 mg L−1 Gibberellic acid (GA3), yielding an average of 14.45 ± 0.57 shoots with an average length of 8.53 ± 1.78 cm. Successful rooting at 94.73 ± 0.37% occurred on half-strength MS medium with 0.5 mg L−1 Indole 3-butyric acid (IBA). In vitro raised plants showed ex vitro survival of 82.55% upon acclimatization. Biochemical analyses revealed that in vitro plants had higher levels of photopigments, phenolics, flavonoids, and antioxidant activity compared to donor plants. Gas chromatography-mass spectrometry (GC-MS) analysis indicated comparatively increased volatile phytochemical content in in vitro plants. Morphological and physiological assessments showed that in vitro plantlets developed larger, greener leaves, lower stomatal density, and higher photosynthetic efficiency than donor plants, supporting enhanced acclimatization and growth performance. This reproducible protocol provides a practical approach for the mass propagation, domestication, and commercial cultivation of I. emodi, facilitating its use as an ornamental and medicinal plant species.