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<p><i>Humulus lupulus</i> is widely used in the brewing industry for its essential oils and bitter compounds, in addition to its applications in pharmaceuticals and cosmetics. This study explores a mixotrophic cultivation methodology using low-cost microporous membranes to facilitate gas exchange rates, aiming to enhance the morphophysiological characteristics of hop plants in vitro. The factors consisted of two sealing systems: with (1&#xa0;M) or without a microporous membrane (0&#xa0;M), and three sucrose concentrations (5, 15, and 30&#xa0;g L<sup>−1</sup>). Morphophysiological parameters were evaluated 45&#xa0;days after in vitro culture. Additionally, acclimatization and survival rates after ex vitro transfer were measured. Plants cultivated under higher gas exchange conditions (1&#xa0;M) and with sucrose concentrations of 15 and 30&#xa0;g L<sup>−1</sup> exhibited significant improvements in growth parameters, including shoot length, leaf area, and root development. Hop plants showed photosynthetic activity when grown with 30&#xa0;g L<sup>−1</sup> sucrose, irrespective of the sealing system. However, at low concentrations activity only occurred with increased gas exchange. The use of membranes facilitated better transpiration regulation, leading to higher survival rates during acclimatization. Plants with microporous membranes exhibited higher photosynthetic pigment content, especially when grown with 15&#xa0;g L<sup>−1</sup> sucrose. Chlorophyll fluorescence analysis demonstrated enhanced PS II performance and energy dissipation in plants cultivated with microporous membranes.</p> Graphical Abstract <p></p>

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Increased gas exchange improves photosynthetic efficiency, growth, and acclimatization in micropropagated hop plants

  • 期刊论文

  • 发表日期：

    2025年6月25日

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  • Ellen Moura Vale,
  • Daniel Pereira Miranda,
  • Wallace de Paula Bernardo,
  • Guilherme Augusto Rodrigues de Souza,
  • Laísa Zanelato Correia,
  • Márcia Helena de Oliveira Martins,
  • Rosana Maria dos Santos Nani de Miranda,
  • Virginia Silva Carvalho,
  • Eliemar Campostrini

摘要

Humulus lupulus is widely used in the brewing industry for its essential oils and bitter compounds, in addition to its applications in pharmaceuticals and cosmetics. This study explores a mixotrophic cultivation methodology using low-cost microporous membranes to facilitate gas exchange rates, aiming to enhance the morphophysiological characteristics of hop plants in vitro. The factors consisted of two sealing systems: with (1 M) or without a microporous membrane (0 M), and three sucrose concentrations (5, 15, and 30 g L−1). Morphophysiological parameters were evaluated 45 days after in vitro culture. Additionally, acclimatization and survival rates after ex vitro transfer were measured. Plants cultivated under higher gas exchange conditions (1 M) and with sucrose concentrations of 15 and 30 g L−1 exhibited significant improvements in growth parameters, including shoot length, leaf area, and root development. Hop plants showed photosynthetic activity when grown with 30 g L−1 sucrose, irrespective of the sealing system. However, at low concentrations activity only occurred with increased gas exchange. The use of membranes facilitated better transpiration regulation, leading to higher survival rates during acclimatization. Plants with microporous membranes exhibited higher photosynthetic pigment content, especially when grown with 15 g L−1 sucrose. Chlorophyll fluorescence analysis demonstrated enhanced PS II performance and energy dissipation in plants cultivated with microporous membranes.

Graphical Abstract