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<p>This study presents an optimized protocol for the ex-situ conservation and micropropagation of <i>Uncaria guianensis</i>, a medicinal plant, using conventional and bioreactor-based tissue culture systems. Seeds were desiccated (0, 24, and 48&#xa0;h) and then stored at–20 ºC or–196 ºC (liquid nitrogen) for up to 365 days, with or without cryoprotectants. In vitro propagation was performed using stem segments cultured in semi-solid, liquid, and bioreactors systems (RITA<sup>®</sup> and Twin Flask– TIS<sup>®</sup>). The most efficient system was further optimized by testing MS and WPM media with 6-benzylaminopurine (BAP) (0–4.0 mg L<sup>−1</sup>) over three subcultures. Micro-cuttings were rooted in MS or WPM media supplemented with indole-3-butyric acid (IBA) (0–1.0 mg L<sup>−1</sup>). Results showed that cryopreserved seeds retained over 70% germination without desiccation or cryoprotectants. The RITA<sup>®</sup> system enhanced shoot multiplication and plant quality. WPM medium promoted greater plantlet height, while BAP (2–4 mg L<sup>−1</sup>) improved multiplication rates. Adventitious root formation reached 90%, but IBA negatively affected rooting. Acclimatized plantlets exhibited 100% survival and stable nuclear DNA content (1.85 pg). This study established an effective approach for the conservation and propagation of <i>Uncaria guianensis</i>.</p>

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Integrated seed cryopreservation, bioreactor and conventional micropropagation, and genetic stability assessment by flow cytometry of Uncaria guianensis (Aubl.) J.F.Gmel, a high-value Amazonian medicinal plant

  • 期刊论文

  • 发表日期：

    2025年4月30日

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  • Hugo Teixeira Gomes,
  • Tatiane Rosa Monteiro,
  • Julceia Camillo,
  • Gabriela Ferreira Nogueira,
  • Frederico Henrique da Silva Costa,
  • Inaê Mariê de Araújo Silva-Cardoso,
  • Jonny Everson Scherwinski-Pereira

摘要

This study presents an optimized protocol for the ex-situ conservation and micropropagation of Uncaria guianensis, a medicinal plant, using conventional and bioreactor-based tissue culture systems. Seeds were desiccated (0, 24, and 48 h) and then stored at–20 ºC or–196 ºC (liquid nitrogen) for up to 365 days, with or without cryoprotectants. In vitro propagation was performed using stem segments cultured in semi-solid, liquid, and bioreactors systems (RITA® and Twin Flask– TIS®). The most efficient system was further optimized by testing MS and WPM media with 6-benzylaminopurine (BAP) (0–4.0 mg L−1) over three subcultures. Micro-cuttings were rooted in MS or WPM media supplemented with indole-3-butyric acid (IBA) (0–1.0 mg L−1). Results showed that cryopreserved seeds retained over 70% germination without desiccation or cryoprotectants. The RITA® system enhanced shoot multiplication and plant quality. WPM medium promoted greater plantlet height, while BAP (2–4 mg L−1) improved multiplication rates. Adventitious root formation reached 90%, but IBA negatively affected rooting. Acclimatized plantlets exhibited 100% survival and stable nuclear DNA content (1.85 pg). This study established an effective approach for the conservation and propagation of Uncaria guianensis.