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<p>Automation in somatic embryogenesis offers a promising solution to meet the demand for high-quality planting material and to reduce production costs compared to current in vitro shoot multiplication techniques. This study presents a novel temporary immersion bioreactor (TIB) system designed specifically for somatic embryogenesis, utilizing embryogenic cell suspensions (ECS) for plant germination. The system demonstrated high efficiency, with one milliliter of settled cell volume (SCV) of embryogenic cells producing 12.54 ± 0.4&#xa0;g of somatic embryos. Subsequently, 300 milligrams of these embryos yielded 2392 ± 60 number of plants in banana. Genetic integrity of the plants was evaluated using flow cytometry and simple sequence repeats (SSR) marker analysis, revealing no variations in nuclear DNA peaks or SSR banding patterns, and field planting showed no adverse effects on vegetative or yield characteristics. Based on these insights, we highlight key challenges, knowledge gaps, technical solutions, research opportunities, and the need for a strategic framework to support industry for the adoption of integrated ECS and TIB technology for commercial production of high quality planting material. Addressing these gaps is essential to promoting industry-wide adoption, enabling cost-effective, large-scale propagation of high-quality planting material for sustainable agriculture and enhanced food security.</p>

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Automation in somatic embryogenesis of banana (Musa spp.) opens new avenue for tissue culture industry

  • 期刊论文

  • 发表日期：

    2025年4月28日

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  • Raju Karthic,
  • Sathiamoorthy Kalpana,
  • Subbaraya Uma,
  • Suthanthiram Backiyarani

摘要

Automation in somatic embryogenesis offers a promising solution to meet the demand for high-quality planting material and to reduce production costs compared to current in vitro shoot multiplication techniques. This study presents a novel temporary immersion bioreactor (TIB) system designed specifically for somatic embryogenesis, utilizing embryogenic cell suspensions (ECS) for plant germination. The system demonstrated high efficiency, with one milliliter of settled cell volume (SCV) of embryogenic cells producing 12.54 ± 0.4 g of somatic embryos. Subsequently, 300 milligrams of these embryos yielded 2392 ± 60 number of plants in banana. Genetic integrity of the plants was evaluated using flow cytometry and simple sequence repeats (SSR) marker analysis, revealing no variations in nuclear DNA peaks or SSR banding patterns, and field planting showed no adverse effects on vegetative or yield characteristics. Based on these insights, we highlight key challenges, knowledge gaps, technical solutions, research opportunities, and the need for a strategic framework to support industry for the adoption of integrated ECS and TIB technology for commercial production of high quality planting material. Addressing these gaps is essential to promoting industry-wide adoption, enabling cost-effective, large-scale propagation of high-quality planting material for sustainable agriculture and enhanced food security.