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<p>Methyl jasmonate elicitation (MeJa) can enhance the steviol glycoside (SG) accumulation in <i>Stevia rebaudiana</i>. The biosynthesis process of SG shares an identical biosynthetic pathway with the gibberellin (GA) hormone. MeJa frequently suppresses GA activity, which is involved in plant growth. This study sought to examine the signaling mechanism of MeJa and its effects on SG and GA, at both physiological and molecular levels, utilizing transcriptomics and gene expression analysis. The physiological analysis showed that MeJa had no impact on the increase of height, leaf area, leaf count, and axillary shoot number on 3&#xa0;days after treatment (DAT). Furthermore, MeJa increased the hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) levels significantly, as well as the levels of stevioside and rebaudioside A. Transcriptomic analysis revealed that 874 genes exhibited differential expression (DEGs) subsequent to MeJa elicitation. Some genes underwent substantial upregulation, including genes associated with the biosynthesis of MeJa, ROS, antioxidant enzyme activity, and secondary metabolite. The gene expression related to SG biosynthesis exhibited an elevation in SrKA13H and SrUGT74G1 gene expression compared to the control. Additionally, there was a significant upregulation in the expression of the Ga2ox1 gene, whereas the Ga3ox gene was downregulated. Our research demonstrated the influence of MeJa elicitation on the biosynthesis of SG and GA in <i>S. rebaudiana.</i> The physiological results were in line with molecular analysis. Ga2ox1 and Ga3ox gene expression might be related to the inactivation of the GA function which affected plant growth, while SrKA13H and SrUGT74G1 gene expression directly increased SG biosynthesis.</p>

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Growth and steviol glycoside analysis on Stevia rebaudiana shoot culture elicited by methyl jasmonate: insight from transcriptomics

  • 期刊论文

  • 发表日期：

    2025年8月11日

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  • Andira Rahmawati,
  • Ahmad Faizal,
  • Iriawati,
  • Roohaida Othman,
  • Husna Nugrahapraja,
  • Rizkita Rachmi Esyanti

摘要

Methyl jasmonate elicitation (MeJa) can enhance the steviol glycoside (SG) accumulation in Stevia rebaudiana. The biosynthesis process of SG shares an identical biosynthetic pathway with the gibberellin (GA) hormone. MeJa frequently suppresses GA activity, which is involved in plant growth. This study sought to examine the signaling mechanism of MeJa and its effects on SG and GA, at both physiological and molecular levels, utilizing transcriptomics and gene expression analysis. The physiological analysis showed that MeJa had no impact on the increase of height, leaf area, leaf count, and axillary shoot number on 3 days after treatment (DAT). Furthermore, MeJa increased the hydrogen peroxide (H2O2) levels significantly, as well as the levels of stevioside and rebaudioside A. Transcriptomic analysis revealed that 874 genes exhibited differential expression (DEGs) subsequent to MeJa elicitation. Some genes underwent substantial upregulation, including genes associated with the biosynthesis of MeJa, ROS, antioxidant enzyme activity, and secondary metabolite. The gene expression related to SG biosynthesis exhibited an elevation in SrKA13H and SrUGT74G1 gene expression compared to the control. Additionally, there was a significant upregulation in the expression of the Ga2ox1 gene, whereas the Ga3ox gene was downregulated. Our research demonstrated the influence of MeJa elicitation on the biosynthesis of SG and GA in S. rebaudiana. The physiological results were in line with molecular analysis. Ga2ox1 and Ga3ox gene expression might be related to the inactivation of the GA function which affected plant growth, while SrKA13H and SrUGT74G1 gene expression directly increased SG biosynthesis.